Mode of action of colicin Ib Formation of ion-permeable membrane channels

Craig A. Weaver; Bruce L. Kagan; Alan Finkelstein; Jordan Konisky

doi:10.1016/0005-2736(81)90521-6

Mode of action of colicin Ib Formation of ion-permeable membrane channels

Craig A. Weaver, Bruce L. Kagan, Alan Finkelstein, Jordan Konisky

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Addition of purified colicin Ib to whole Escherichia coli cells or cytoplasmic membrane vesicles inhibits their subsequent ability to generate a membrane potential. In addition, this colicin is shown to bring about a voltage-dependent increase in the conductance of an artificial planar bilayer membrane prepared from soybean phospholipids. This results from the formation of ion-permeable channels. These data provide strong evidence that the depolarization of Escherichia coli cells by this colicin results from an Ib-induced increase in membrane permeability to ions.

Original language	English (US)
Pages (from-to)	137-142
Number of pages	6
Journal	BBA - Biomembranes
Volume	645
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(81)90521-6
State	Published - Jun 6 1981
Externally published	Yes

Keywords

(E. coli, Planar bilayer, Vesicle)
Colicin Ib
Ion channel
Ion permeability
Membrane potential

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(81)90521-6

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title = "Mode of action of colicin Ib Formation of ion-permeable membrane channels",

abstract = "Addition of purified colicin Ib to whole Escherichia coli cells or cytoplasmic membrane vesicles inhibits their subsequent ability to generate a membrane potential. In addition, this colicin is shown to bring about a voltage-dependent increase in the conductance of an artificial planar bilayer membrane prepared from soybean phospholipids. This results from the formation of ion-permeable channels. These data provide strong evidence that the depolarization of Escherichia coli cells by this colicin results from an Ib-induced increase in membrane permeability to ions.",

keywords = "(E. coli, Planar bilayer, Vesicle), Colicin Ib, Ion channel, Ion permeability, Membrane potential",

author = "Weaver, {Craig A.} and Kagan, {Bruce L.} and Alan Finkelstein and Jordan Konisky",

note = "Funding Information: This research was supported by Public Health Service grants AI 10106 (J.K. and C.W.) and National Institutes of Health grants 5T 32 GM 7288 and NS 14246-03 (B.L.K. and A.F.). Jordan Konisky was the recipient of PHS Research Development Award K04-AI 100049. Craig A. Weaver is a recipient of National Institutes of Health Predoctoral Traineeship GM 7288.",

year = "1981",

month = jun,

day = "6",

doi = "10.1016/0005-2736(81)90521-6",

language = "English (US)",

volume = "645",

pages = "137--142",

journal = "Biochimica et Biophysica Acta - Biomembranes",

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publisher = "Elsevier",

number = "1",

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T1 - Mode of action of colicin Ib Formation of ion-permeable membrane channels

AU - Weaver, Craig A.

AU - Kagan, Bruce L.

AU - Finkelstein, Alan

AU - Konisky, Jordan

N1 - Funding Information: This research was supported by Public Health Service grants AI 10106 (J.K. and C.W.) and National Institutes of Health grants 5T 32 GM 7288 and NS 14246-03 (B.L.K. and A.F.). Jordan Konisky was the recipient of PHS Research Development Award K04-AI 100049. Craig A. Weaver is a recipient of National Institutes of Health Predoctoral Traineeship GM 7288.

PY - 1981/6/6

Y1 - 1981/6/6

N2 - Addition of purified colicin Ib to whole Escherichia coli cells or cytoplasmic membrane vesicles inhibits their subsequent ability to generate a membrane potential. In addition, this colicin is shown to bring about a voltage-dependent increase in the conductance of an artificial planar bilayer membrane prepared from soybean phospholipids. This results from the formation of ion-permeable channels. These data provide strong evidence that the depolarization of Escherichia coli cells by this colicin results from an Ib-induced increase in membrane permeability to ions.

AB - Addition of purified colicin Ib to whole Escherichia coli cells or cytoplasmic membrane vesicles inhibits their subsequent ability to generate a membrane potential. In addition, this colicin is shown to bring about a voltage-dependent increase in the conductance of an artificial planar bilayer membrane prepared from soybean phospholipids. This results from the formation of ion-permeable channels. These data provide strong evidence that the depolarization of Escherichia coli cells by this colicin results from an Ib-induced increase in membrane permeability to ions.

KW - (E. coli, Planar bilayer, Vesicle)

KW - Colicin Ib

KW - Ion channel

KW - Ion permeability

KW - Membrane potential

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AN - SCOPUS:0019464086

SN - 0005-2736

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JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

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ER -

Mode of action of colicin Ib Formation of ion-permeable membrane channels

Abstract

Keywords

ASJC Scopus subject areas

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