Mode of action of colicin Ib Formation of ion-permeable membrane channels

Craig A. Weaver, Bruce L. Kagan, Alan Finkelstein, Jordan Konisky

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Addition of purified colicin Ib to whole Escherichia coli cells or cytoplasmic membrane vesicles inhibits their subsequent ability to generate a membrane potential. In addition, this colicin is shown to bring about a voltage-dependent increase in the conductance of an artificial planar bilayer membrane prepared from soybean phospholipids. This results from the formation of ion-permeable channels. These data provide strong evidence that the depolarization of Escherichia coli cells by this colicin results from an Ib-induced increase in membrane permeability to ions.

Original languageEnglish (US)
Pages (from-to)137-142
Number of pages6
JournalBBA - Biomembranes
Volume645
Issue number1
DOIs
StatePublished - Jun 6 1981

Fingerprint

Colicins
Ion Channels
Ions
Membranes
Cell Membrane
Cytoplasmic Vesicles
Escherichia coli
Soybeans
Membrane Potentials
Permeability
Phospholipids
Depolarization
Electric potential

Keywords

  • (E. coli, Planar bilayer, Vesicle)
  • Colicin Ib
  • Ion channel
  • Ion permeability
  • Membrane potential

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

Mode of action of colicin Ib Formation of ion-permeable membrane channels. / Weaver, Craig A.; Kagan, Bruce L.; Finkelstein, Alan; Konisky, Jordan.

In: BBA - Biomembranes, Vol. 645, No. 1, 06.06.1981, p. 137-142.

Research output: Contribution to journalArticle

Weaver, CA, Kagan, BL, Finkelstein, A & Konisky, J 1981, 'Mode of action of colicin Ib Formation of ion-permeable membrane channels', BBA - Biomembranes, vol. 645, no. 1, pp. 137-142. https://doi.org/10.1016/0005-2736(81)90521-6
Weaver, Craig A. ; Kagan, Bruce L. ; Finkelstein, Alan ; Konisky, Jordan. / Mode of action of colicin Ib Formation of ion-permeable membrane channels. In: BBA - Biomembranes. 1981 ; Vol. 645, No. 1. pp. 137-142.
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