Mobilization of an ERV9 Human Endogenous Retroviral Element during Primate Evolution

Antonio Di Cristofano, Maria Strazzullo, Tiziana Parisi, Girolama La Mantia

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

ERV9 is a low repeated family of human endogenous retroviral elements, which has close to 50 members, in addition to at least 4000 solitary LTRs. Previous work has shown that randomly selected LTRs can promote transcription of reporter genes, raising the possibility that these sequences may affect the expression of adjacent cellular genes. We report here the structural organization in different primate species of a zinc-finger coding gene whose expression is driven in humans by a solitary ERV9-LTR promoter. Using a PCR strategy and library screening, we were able to trace the origin of the insertion event in the primate lineage and to evaluate the impact of this event on gene structure. Our findings indicate that the integration of the ERV9 element occurred after the split of orangutang from the great apes, but before the divergence of the gorilla lineage. These results suggest that ERV9 elements have been mobile within the primate lineages and may still be active in humans.

Original languageEnglish (US)
Pages (from-to)271-275
Number of pages5
JournalVirology
Volume213
Issue number1
DOIs
StatePublished - Oct 20 1995
Externally publishedYes

Fingerprint

Primates
Gorilla gorilla
Zinc Fingers
Hominidae
Reporter Genes
Genes
Gene Expression
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Mobilization of an ERV9 Human Endogenous Retroviral Element during Primate Evolution. / Di Cristofano, Antonio; Strazzullo, Maria; Parisi, Tiziana; La Mantia, Girolama.

In: Virology, Vol. 213, No. 1, 20.10.1995, p. 271-275.

Research output: Contribution to journalArticle

Di Cristofano, Antonio ; Strazzullo, Maria ; Parisi, Tiziana ; La Mantia, Girolama. / Mobilization of an ERV9 Human Endogenous Retroviral Element during Primate Evolution. In: Virology. 1995 ; Vol. 213, No. 1. pp. 271-275.
@article{953ded2181534d79b7dc1e408636bc59,
title = "Mobilization of an ERV9 Human Endogenous Retroviral Element during Primate Evolution",
abstract = "ERV9 is a low repeated family of human endogenous retroviral elements, which has close to 50 members, in addition to at least 4000 solitary LTRs. Previous work has shown that randomly selected LTRs can promote transcription of reporter genes, raising the possibility that these sequences may affect the expression of adjacent cellular genes. We report here the structural organization in different primate species of a zinc-finger coding gene whose expression is driven in humans by a solitary ERV9-LTR promoter. Using a PCR strategy and library screening, we were able to trace the origin of the insertion event in the primate lineage and to evaluate the impact of this event on gene structure. Our findings indicate that the integration of the ERV9 element occurred after the split of orangutang from the great apes, but before the divergence of the gorilla lineage. These results suggest that ERV9 elements have been mobile within the primate lineages and may still be active in humans.",
author = "{Di Cristofano}, Antonio and Maria Strazzullo and Tiziana Parisi and {La Mantia}, Girolama",
year = "1995",
month = "10",
day = "20",
doi = "10.1006/viro.1995.1570",
language = "English (US)",
volume = "213",
pages = "271--275",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Mobilization of an ERV9 Human Endogenous Retroviral Element during Primate Evolution

AU - Di Cristofano, Antonio

AU - Strazzullo, Maria

AU - Parisi, Tiziana

AU - La Mantia, Girolama

PY - 1995/10/20

Y1 - 1995/10/20

N2 - ERV9 is a low repeated family of human endogenous retroviral elements, which has close to 50 members, in addition to at least 4000 solitary LTRs. Previous work has shown that randomly selected LTRs can promote transcription of reporter genes, raising the possibility that these sequences may affect the expression of adjacent cellular genes. We report here the structural organization in different primate species of a zinc-finger coding gene whose expression is driven in humans by a solitary ERV9-LTR promoter. Using a PCR strategy and library screening, we were able to trace the origin of the insertion event in the primate lineage and to evaluate the impact of this event on gene structure. Our findings indicate that the integration of the ERV9 element occurred after the split of orangutang from the great apes, but before the divergence of the gorilla lineage. These results suggest that ERV9 elements have been mobile within the primate lineages and may still be active in humans.

AB - ERV9 is a low repeated family of human endogenous retroviral elements, which has close to 50 members, in addition to at least 4000 solitary LTRs. Previous work has shown that randomly selected LTRs can promote transcription of reporter genes, raising the possibility that these sequences may affect the expression of adjacent cellular genes. We report here the structural organization in different primate species of a zinc-finger coding gene whose expression is driven in humans by a solitary ERV9-LTR promoter. Using a PCR strategy and library screening, we were able to trace the origin of the insertion event in the primate lineage and to evaluate the impact of this event on gene structure. Our findings indicate that the integration of the ERV9 element occurred after the split of orangutang from the great apes, but before the divergence of the gorilla lineage. These results suggest that ERV9 elements have been mobile within the primate lineages and may still be active in humans.

UR - http://www.scopus.com/inward/record.url?scp=0028880713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028880713&partnerID=8YFLogxK

U2 - 10.1006/viro.1995.1570

DO - 10.1006/viro.1995.1570

M3 - Article

VL - 213

SP - 271

EP - 275

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -