MLL tandem duplication and multiple splicing in adult acute myeloid leukemia with normal karyotype

M. Yu, K. Honoki, J. Andersen, Elisabeth M. Paietta, D. K. Nam, J. J. Yunis

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Rearrangement of the MLL (myeloid-lymphoid or mixed-lineage leukemia) gene through a reciprocal chromosomal translocation is found in 5% of adult acute myeloid (AML) and 10% of pediatric acute lymphoid (ALL) leukemia. More than 25 different reciprocal chromosomal translocations, with an 11q23 breakpoint, fuse the MLL gene (also named ALL-1, HRX and Htrx1) to a second partner gene. These leukemias have poor prognosis and frequently have a monocytic, lymphoid or biphenotypic (myeloid and lymphoid) antigen expression in blast cells. Approximately 20-30% of patients diagnosed as having adult de novo AML have normal chromosomes by metaphase analysis and the majority of these patients have good prognosis. With the use of reverse transcriptase-polymerase chain reaction (RT-PCR) technique and Southern blot analysis, we found that seven of 34 such patients (21%) had a tandem partial duplication of exons 2 to 6 or 2 to 8 of the MLL gene. These seven patients showed a median survival of 2.7 months, compared to a 6.8 months median survival for all other patients in the study. If confirmed on a large series of patients, our findings may help differentiate AML with normal karyotype and poor prognosis from those with normal karyotype and a more favorable prognosis.

Original languageEnglish (US)
Pages (from-to)774-780
Number of pages7
JournalLeukemia
Volume10
Issue number5
StatePublished - May 1996

Fingerprint

Karyotype
Acute Myeloid Leukemia
Leukemia
Genetic Translocation
Genes
Survival
Metaphase
Southern Blotting
Reverse Transcriptase Polymerase Chain Reaction
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Exons
Chromosomes
Pediatrics
Antigens

Keywords

  • Acute leukemia
  • Karyotype
  • MLL
  • Prognosis
  • Tandem duplication

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Yu, M., Honoki, K., Andersen, J., Paietta, E. M., Nam, D. K., & Yunis, J. J. (1996). MLL tandem duplication and multiple splicing in adult acute myeloid leukemia with normal karyotype. Leukemia, 10(5), 774-780.

MLL tandem duplication and multiple splicing in adult acute myeloid leukemia with normal karyotype. / Yu, M.; Honoki, K.; Andersen, J.; Paietta, Elisabeth M.; Nam, D. K.; Yunis, J. J.

In: Leukemia, Vol. 10, No. 5, 05.1996, p. 774-780.

Research output: Contribution to journalArticle

Yu, M, Honoki, K, Andersen, J, Paietta, EM, Nam, DK & Yunis, JJ 1996, 'MLL tandem duplication and multiple splicing in adult acute myeloid leukemia with normal karyotype', Leukemia, vol. 10, no. 5, pp. 774-780.
Yu, M. ; Honoki, K. ; Andersen, J. ; Paietta, Elisabeth M. ; Nam, D. K. ; Yunis, J. J. / MLL tandem duplication and multiple splicing in adult acute myeloid leukemia with normal karyotype. In: Leukemia. 1996 ; Vol. 10, No. 5. pp. 774-780.
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