ML antigen of DBA/2 mouse leukemias: Expression of an endogenous murine mammary tumor virus

Janis Racevskis, N. H. Sarkar

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Spontaneous, transplantable leukemias of DBA/2 mice express an antigen (ML) which cross-reacts with antigens of murine mammary tumor virus (muMTV). The MuMTV cross-reactive antigen of the DBA/2 leukemias (ML cells) was found to be a glycoprotein of 78,000 molecular weight containing antigenic determinants of the major MuMTV glycoprotein gp52. No MuMTV particles were produced by the ML cells, although they did contain type A particles - the pronucleocapsids of MuMTV. The ML antigen appeared to be an aberrant form of the intracellular MuMTV env precursor molecule prgp70, which was not processed properly but instead acquired extra carbohydrate groups and was expressed in uncleaved form on the cell surface. Isolation of MuMTV core protein p28 from the leukemic cells and subsequent tryptic peptide mapping analysis showed that the p28 from leukemia cells differed from the p28 of MuMTV isolated from DBA/2 mouse milk. These observations indicate that the MuMTV expressed in DBA/2 leukemic spleen cells is of a different strain than the virus secreted in lactating mammary glands of DBA/2 mice and probably represents the expression of an endogenous DBA/2 provirus.

Original languageEnglish (US)
Pages (from-to)804-813
Number of pages10
JournalJournal of Virology
Volume42
Issue number3
StatePublished - 1982
Externally publishedYes

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Gammaretrovirus
Inbred DBA Mouse
mammary neoplasms (animal)
leukemia
Leukemia
Breast Neoplasms
antigens
Antigens
viruses
mice
cells
glycoproteins
Glycoproteins
proviruses
peptide mapping
Proviruses
splenocytes
Peptide Mapping
mammary glands
epitopes

ASJC Scopus subject areas

  • Immunology

Cite this

ML antigen of DBA/2 mouse leukemias : Expression of an endogenous murine mammary tumor virus. / Racevskis, Janis; Sarkar, N. H.

In: Journal of Virology, Vol. 42, No. 3, 1982, p. 804-813.

Research output: Contribution to journalArticle

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