TY - JOUR
T1 - Mixed culture and differentiation of two samples of human umbilical cord blood-derived mesenchymal stem cells into neuron-like cells
AU - Jiao, Hong Liang
AU - Yang, Bo
AU - Guan, Fang Xia
AU - Li, Jian Bin
AU - Du, Ying
AU - San, Hong
AU - Hu, Xiang
AU - Hu, Wei
AU - Ma, Jian
AU - Man, Yong
AU - Zhao, Lin Na
AU - Duan, Yan Li
PY - 2008/5/20
Y1 - 2008/5/20
N2 - Background: Umbilical cord blood mesenchymal stem cells (UCBMSCs) can differentiate into nerve-like cells, but number of stem cell proliferation was not enough in clinic. Objective: To investigate the mixed culture growth of two samples of UCBMSCs, and the differentation of UCBMSCs into neuron-like cells after induced by basic fibroblast growth factor (bFGF). Design, Time and Setting: A control observational cell study was performed at the Laboratory of Department of Microbiology and Immunology of Basic Medical College of Zhengzhou University from January to October in 2007. Materials: 60-180 mL of umbilical cord blood was collected from healthy voluntary puerperants. Methods: Human UCBMSCs was harvested with Ficoll-Hypaque lymphocyte isolating medium and by density gradient centrifugation, subsequently, induced by bFGF. To obtain 3rd passage cells, the six samples of UCBMSCs were labelled by random as group A (n=6, half). Another six samples of UCBMSCs were labelled as group B (n=6, half). The six mixed UCBMSCs were labelled as group C (n=6, surplus parts of groups A and B). Cell density in the three groups was adjusted to 1.0 × 104 L-1. Main Outcome Measures: Amount of cell proliferation was compared by counting living cells and MTT colorimetry. Nerval marker of glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE) and nestin expressions in UCBMSCs were detected by immunohistochemistry. Results: Adherence of the mixed UCBMSCs was faster than the single by counting cell number and MTT colorimetry (P < 0.05), so did the proliferation of mixed cells (P < 0.05). UCBMSCs induced by bFGF displayed positive immunoreactivity to nerval marker of GFAP, NSE and nestin. There was no significant difference among three groups in percentage of nestin expression (P > 0.05). Conclusion: The mixed MSCs promote proliferation each other. MSCs induced by bFGF express positive immunoreactivity to nerval marker of GFAP, NSE and nestin.
AB - Background: Umbilical cord blood mesenchymal stem cells (UCBMSCs) can differentiate into nerve-like cells, but number of stem cell proliferation was not enough in clinic. Objective: To investigate the mixed culture growth of two samples of UCBMSCs, and the differentation of UCBMSCs into neuron-like cells after induced by basic fibroblast growth factor (bFGF). Design, Time and Setting: A control observational cell study was performed at the Laboratory of Department of Microbiology and Immunology of Basic Medical College of Zhengzhou University from January to October in 2007. Materials: 60-180 mL of umbilical cord blood was collected from healthy voluntary puerperants. Methods: Human UCBMSCs was harvested with Ficoll-Hypaque lymphocyte isolating medium and by density gradient centrifugation, subsequently, induced by bFGF. To obtain 3rd passage cells, the six samples of UCBMSCs were labelled by random as group A (n=6, half). Another six samples of UCBMSCs were labelled as group B (n=6, half). The six mixed UCBMSCs were labelled as group C (n=6, surplus parts of groups A and B). Cell density in the three groups was adjusted to 1.0 × 104 L-1. Main Outcome Measures: Amount of cell proliferation was compared by counting living cells and MTT colorimetry. Nerval marker of glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE) and nestin expressions in UCBMSCs were detected by immunohistochemistry. Results: Adherence of the mixed UCBMSCs was faster than the single by counting cell number and MTT colorimetry (P < 0.05), so did the proliferation of mixed cells (P < 0.05). UCBMSCs induced by bFGF displayed positive immunoreactivity to nerval marker of GFAP, NSE and nestin. There was no significant difference among three groups in percentage of nestin expression (P > 0.05). Conclusion: The mixed MSCs promote proliferation each other. MSCs induced by bFGF express positive immunoreactivity to nerval marker of GFAP, NSE and nestin.
UR - http://www.scopus.com/inward/record.url?scp=48949118705&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48949118705&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:48949118705
SN - 1673-8225
VL - 12
SP - 4021
EP - 4025
JO - Journal of Clinical Rehabilitative Tissue Engineering Research
JF - Journal of Clinical Rehabilitative Tissue Engineering Research
IS - 21
ER -
