Mixed culture and differentiation of two samples of human umbilical cord blood-derived mesenchymal stem cells into neuron-like cells

Hong Liang Jiao, Bo Yang, Fangxia Guan, Jian Bin Li, Ying Du, Hong San, Xiang Hu, Wei Hu, Jian Ma, Yong Man, Lin Na Zhao, Yan Li Duan

Research output: Contribution to journalArticle

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Abstract

Background: Umbilical cord blood mesenchymal stem cells (UCBMSCs) can differentiate into nerve-like cells, but number of stem cell proliferation was not enough in clinic. Objective: To investigate the mixed culture growth of two samples of UCBMSCs, and the differentation of UCBMSCs into neuron-like cells after induced by basic fibroblast growth factor (bFGF). Design, Time and Setting: A control observational cell study was performed at the Laboratory of Department of Microbiology and Immunology of Basic Medical College of Zhengzhou University from January to October in 2007. Materials: 60-180 mL of umbilical cord blood was collected from healthy voluntary puerperants. Methods: Human UCBMSCs was harvested with Ficoll-Hypaque lymphocyte isolating medium and by density gradient centrifugation, subsequently, induced by bFGF. To obtain 3rd passage cells, the six samples of UCBMSCs were labelled by random as group A (n=6, half). Another six samples of UCBMSCs were labelled as group B (n=6, half). The six mixed UCBMSCs were labelled as group C (n=6, surplus parts of groups A and B). Cell density in the three groups was adjusted to 1.0 × 104 L-1. Main Outcome Measures: Amount of cell proliferation was compared by counting living cells and MTT colorimetry. Nerval marker of glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE) and nestin expressions in UCBMSCs were detected by immunohistochemistry. Results: Adherence of the mixed UCBMSCs was faster than the single by counting cell number and MTT colorimetry (P < 0.05), so did the proliferation of mixed cells (P < 0.05). UCBMSCs induced by bFGF displayed positive immunoreactivity to nerval marker of GFAP, NSE and nestin. There was no significant difference among three groups in percentage of nestin expression (P > 0.05). Conclusion: The mixed MSCs promote proliferation each other. MSCs induced by bFGF express positive immunoreactivity to nerval marker of GFAP, NSE and nestin.

Original languageEnglish (US)
Pages (from-to)4021-4025
Number of pages5
JournalJournal of Clinical Rehabilitative Tissue Engineering Research
Volume12
Issue number21
StatePublished - May 20 2008
Externally publishedYes

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Stem cells
Mesenchymal Stromal Cells
Fetal Blood
Neurons
Blood
Fibroblast Growth Factor 2
Fibroblasts
Colorimetry
Nestin
Phosphopyruvate Hydratase
Cell Count
Glial Fibrillary Acidic Protein
Cell proliferation
Cell Proliferation
Immunology
Diatrizoate
Proteins
Microbiology
Ficoll
Lymphocytes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biomedical Engineering
  • Transplantation

Cite this

Mixed culture and differentiation of two samples of human umbilical cord blood-derived mesenchymal stem cells into neuron-like cells. / Jiao, Hong Liang; Yang, Bo; Guan, Fangxia; Li, Jian Bin; Du, Ying; San, Hong; Hu, Xiang; Hu, Wei; Ma, Jian; Man, Yong; Zhao, Lin Na; Duan, Yan Li.

In: Journal of Clinical Rehabilitative Tissue Engineering Research, Vol. 12, No. 21, 20.05.2008, p. 4021-4025.

Research output: Contribution to journalArticle

Jiao, HL, Yang, B, Guan, F, Li, JB, Du, Y, San, H, Hu, X, Hu, W, Ma, J, Man, Y, Zhao, LN & Duan, YL 2008, 'Mixed culture and differentiation of two samples of human umbilical cord blood-derived mesenchymal stem cells into neuron-like cells', Journal of Clinical Rehabilitative Tissue Engineering Research, vol. 12, no. 21, pp. 4021-4025.
Jiao, Hong Liang ; Yang, Bo ; Guan, Fangxia ; Li, Jian Bin ; Du, Ying ; San, Hong ; Hu, Xiang ; Hu, Wei ; Ma, Jian ; Man, Yong ; Zhao, Lin Na ; Duan, Yan Li. / Mixed culture and differentiation of two samples of human umbilical cord blood-derived mesenchymal stem cells into neuron-like cells. In: Journal of Clinical Rehabilitative Tissue Engineering Research. 2008 ; Vol. 12, No. 21. pp. 4021-4025.
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abstract = "Background: Umbilical cord blood mesenchymal stem cells (UCBMSCs) can differentiate into nerve-like cells, but number of stem cell proliferation was not enough in clinic. Objective: To investigate the mixed culture growth of two samples of UCBMSCs, and the differentation of UCBMSCs into neuron-like cells after induced by basic fibroblast growth factor (bFGF). Design, Time and Setting: A control observational cell study was performed at the Laboratory of Department of Microbiology and Immunology of Basic Medical College of Zhengzhou University from January to October in 2007. Materials: 60-180 mL of umbilical cord blood was collected from healthy voluntary puerperants. Methods: Human UCBMSCs was harvested with Ficoll-Hypaque lymphocyte isolating medium and by density gradient centrifugation, subsequently, induced by bFGF. To obtain 3rd passage cells, the six samples of UCBMSCs were labelled by random as group A (n=6, half). Another six samples of UCBMSCs were labelled as group B (n=6, half). The six mixed UCBMSCs were labelled as group C (n=6, surplus parts of groups A and B). Cell density in the three groups was adjusted to 1.0 × 104 L-1. Main Outcome Measures: Amount of cell proliferation was compared by counting living cells and MTT colorimetry. Nerval marker of glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE) and nestin expressions in UCBMSCs were detected by immunohistochemistry. Results: Adherence of the mixed UCBMSCs was faster than the single by counting cell number and MTT colorimetry (P < 0.05), so did the proliferation of mixed cells (P < 0.05). UCBMSCs induced by bFGF displayed positive immunoreactivity to nerval marker of GFAP, NSE and nestin. There was no significant difference among three groups in percentage of nestin expression (P > 0.05). Conclusion: The mixed MSCs promote proliferation each other. MSCs induced by bFGF express positive immunoreactivity to nerval marker of GFAP, NSE and nestin.",
author = "Jiao, {Hong Liang} and Bo Yang and Fangxia Guan and Li, {Jian Bin} and Ying Du and Hong San and Xiang Hu and Wei Hu and Jian Ma and Yong Man and Zhao, {Lin Na} and Duan, {Yan Li}",
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T1 - Mixed culture and differentiation of two samples of human umbilical cord blood-derived mesenchymal stem cells into neuron-like cells

AU - Jiao, Hong Liang

AU - Yang, Bo

AU - Guan, Fangxia

AU - Li, Jian Bin

AU - Du, Ying

AU - San, Hong

AU - Hu, Xiang

AU - Hu, Wei

AU - Ma, Jian

AU - Man, Yong

AU - Zhao, Lin Na

AU - Duan, Yan Li

PY - 2008/5/20

Y1 - 2008/5/20

N2 - Background: Umbilical cord blood mesenchymal stem cells (UCBMSCs) can differentiate into nerve-like cells, but number of stem cell proliferation was not enough in clinic. Objective: To investigate the mixed culture growth of two samples of UCBMSCs, and the differentation of UCBMSCs into neuron-like cells after induced by basic fibroblast growth factor (bFGF). Design, Time and Setting: A control observational cell study was performed at the Laboratory of Department of Microbiology and Immunology of Basic Medical College of Zhengzhou University from January to October in 2007. Materials: 60-180 mL of umbilical cord blood was collected from healthy voluntary puerperants. Methods: Human UCBMSCs was harvested with Ficoll-Hypaque lymphocyte isolating medium and by density gradient centrifugation, subsequently, induced by bFGF. To obtain 3rd passage cells, the six samples of UCBMSCs were labelled by random as group A (n=6, half). Another six samples of UCBMSCs were labelled as group B (n=6, half). The six mixed UCBMSCs were labelled as group C (n=6, surplus parts of groups A and B). Cell density in the three groups was adjusted to 1.0 × 104 L-1. Main Outcome Measures: Amount of cell proliferation was compared by counting living cells and MTT colorimetry. Nerval marker of glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE) and nestin expressions in UCBMSCs were detected by immunohistochemistry. Results: Adherence of the mixed UCBMSCs was faster than the single by counting cell number and MTT colorimetry (P < 0.05), so did the proliferation of mixed cells (P < 0.05). UCBMSCs induced by bFGF displayed positive immunoreactivity to nerval marker of GFAP, NSE and nestin. There was no significant difference among three groups in percentage of nestin expression (P > 0.05). Conclusion: The mixed MSCs promote proliferation each other. MSCs induced by bFGF express positive immunoreactivity to nerval marker of GFAP, NSE and nestin.

AB - Background: Umbilical cord blood mesenchymal stem cells (UCBMSCs) can differentiate into nerve-like cells, but number of stem cell proliferation was not enough in clinic. Objective: To investigate the mixed culture growth of two samples of UCBMSCs, and the differentation of UCBMSCs into neuron-like cells after induced by basic fibroblast growth factor (bFGF). Design, Time and Setting: A control observational cell study was performed at the Laboratory of Department of Microbiology and Immunology of Basic Medical College of Zhengzhou University from January to October in 2007. Materials: 60-180 mL of umbilical cord blood was collected from healthy voluntary puerperants. Methods: Human UCBMSCs was harvested with Ficoll-Hypaque lymphocyte isolating medium and by density gradient centrifugation, subsequently, induced by bFGF. To obtain 3rd passage cells, the six samples of UCBMSCs were labelled by random as group A (n=6, half). Another six samples of UCBMSCs were labelled as group B (n=6, half). The six mixed UCBMSCs were labelled as group C (n=6, surplus parts of groups A and B). Cell density in the three groups was adjusted to 1.0 × 104 L-1. Main Outcome Measures: Amount of cell proliferation was compared by counting living cells and MTT colorimetry. Nerval marker of glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE) and nestin expressions in UCBMSCs were detected by immunohistochemistry. Results: Adherence of the mixed UCBMSCs was faster than the single by counting cell number and MTT colorimetry (P < 0.05), so did the proliferation of mixed cells (P < 0.05). UCBMSCs induced by bFGF displayed positive immunoreactivity to nerval marker of GFAP, NSE and nestin. There was no significant difference among three groups in percentage of nestin expression (P > 0.05). Conclusion: The mixed MSCs promote proliferation each other. MSCs induced by bFGF express positive immunoreactivity to nerval marker of GFAP, NSE and nestin.

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