Mixed culture and differentiation of two samples of human umbilical cord blood-derived mesenchymal stem cells into neuron-like cells

Hong Liang Jiao, Bo Yang, Fang Xia Guan, Jian Bin Li, Ying Du, Hong San, Xiang Hu, Wei Hu, Jian Ma, Yong Man, Lin Na Zhao, Yan Li Duan

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1 Scopus citations

Abstract

Background: Umbilical cord blood mesenchymal stem cells (UCBMSCs) can differentiate into nerve-like cells, but number of stem cell proliferation was not enough in clinic. Objective: To investigate the mixed culture growth of two samples of UCBMSCs, and the differentation of UCBMSCs into neuron-like cells after induced by basic fibroblast growth factor (bFGF). Design, Time and Setting: A control observational cell study was performed at the Laboratory of Department of Microbiology and Immunology of Basic Medical College of Zhengzhou University from January to October in 2007. Materials: 60-180 mL of umbilical cord blood was collected from healthy voluntary puerperants. Methods: Human UCBMSCs was harvested with Ficoll-Hypaque lymphocyte isolating medium and by density gradient centrifugation, subsequently, induced by bFGF. To obtain 3rd passage cells, the six samples of UCBMSCs were labelled by random as group A (n=6, half). Another six samples of UCBMSCs were labelled as group B (n=6, half). The six mixed UCBMSCs were labelled as group C (n=6, surplus parts of groups A and B). Cell density in the three groups was adjusted to 1.0 × 104 L-1. Main Outcome Measures: Amount of cell proliferation was compared by counting living cells and MTT colorimetry. Nerval marker of glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE) and nestin expressions in UCBMSCs were detected by immunohistochemistry. Results: Adherence of the mixed UCBMSCs was faster than the single by counting cell number and MTT colorimetry (P < 0.05), so did the proliferation of mixed cells (P < 0.05). UCBMSCs induced by bFGF displayed positive immunoreactivity to nerval marker of GFAP, NSE and nestin. There was no significant difference among three groups in percentage of nestin expression (P > 0.05). Conclusion: The mixed MSCs promote proliferation each other. MSCs induced by bFGF express positive immunoreactivity to nerval marker of GFAP, NSE and nestin.

Original languageEnglish (US)
Pages (from-to)4021-4025
Number of pages5
JournalJournal of Clinical Rehabilitative Tissue Engineering Research
Volume12
Issue number21
StatePublished - May 20 2008

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Clinical Biochemistry

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    Jiao, H. L., Yang, B., Guan, F. X., Li, J. B., Du, Y., San, H., Hu, X., Hu, W., Ma, J., Man, Y., Zhao, L. N., & Duan, Y. L. (2008). Mixed culture and differentiation of two samples of human umbilical cord blood-derived mesenchymal stem cells into neuron-like cells. Journal of Clinical Rehabilitative Tissue Engineering Research, 12(21), 4021-4025.