Mitochondrial contagion induced by parkin deficiency in drosophila hearts and its containment by suppressing mitofusin

Poonam Bhandari, Moshi Song, Yun Chen, Yan Burelle, Gerald W. Dorn

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Rationale: Dysfunctional Parkin-mediated mitophagic culling of senescent or damaged mitochondria is a major pathological process underlying Parkinson disease and a potential genetic mechanism of cardiomyopathy. Despite epidemiological associations between Parkinson disease and heart failure, the role of Parkin and mitophagic quality control in maintaining normal cardiac homeostasis is poorly understood. Objective: We used germline mutants and cardiac-specific RNA interference to interrogate Parkin regulation of cardiomyocyte mitochondria and examine functional crosstalk between mitophagy and mitochondrial dynamics in Drosophila heart tubes. Methods and Results: Transcriptional profiling of Parkin knockout mouse hearts revealed compensatory upregulation of multiple related E3 ubiquitin ligases. Because Drosophila lack most of these redundant genes, we examined heart tubes of parkin knockout flies and observed accumulation of enlarged hollow donut mitochondria with dilated cardiomyopathy, which could be rescued by cardiomyocyte-specific Parkin expression. Identical abnormalities were induced by cardiomyocyte-specific Parkin suppression using 2 different inhibitory RNAs. Parkin-deficient cardiomyocyte mitochondria exhibited dysmorphology, depolarization, and reactive oxygen species generation without calcium cycling abnormalities, pointing to a primary mitochondrial defect. Suppressing cardiomyocyte mitochondrial fusion in Parkin-deficient fly heart tubes completely prevented the cardiomyopathy and corrected mitochondrial dysfunction without normalizing mitochondrial dysmorphology, demonstrating a central role for mitochondrial fusion in the cardiomyopathy provoked by impaired mitophagy. Conclusions: Parkin deficiency and resulting mitophagic disruption produces cardiomyopathy in part by contamination of the cardiomyocyte mitochondrial pool through fusion between improperly retained dysfunctional/ senescent and normal mitochondria. Limiting mitochondrial contagion by inhibiting organelle fusion shows promise for minimizing organ dysfunction produced by defective mitophagic signaling.

Original languageEnglish (US)
Pages (from-to)257-265
Number of pages9
JournalCirculation Research
Volume114
Issue number2
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Cardiac Myocytes
Drosophila
Mitochondrial Dynamics
Mitochondria
Cardiomyopathies
Mitochondrial Degradation
Diptera
Parkinson Disease
Ubiquitin-Protein Ligases
Dilated Cardiomyopathy
Pathologic Processes
RNA Interference
Knockout Mice
Quality Control
Organelles
Reactive Oxygen Species
Homeostasis
Up-Regulation
Heart Failure
RNA

Keywords

  • Cardiomyopathies
  • Mitochondrial degradation
  • Mitochondrial dynamics

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Mitochondrial contagion induced by parkin deficiency in drosophila hearts and its containment by suppressing mitofusin. / Bhandari, Poonam; Song, Moshi; Chen, Yun; Burelle, Yan; Dorn, Gerald W.

In: Circulation Research, Vol. 114, No. 2, 2014, p. 257-265.

Research output: Contribution to journalArticle

Bhandari, Poonam ; Song, Moshi ; Chen, Yun ; Burelle, Yan ; Dorn, Gerald W. / Mitochondrial contagion induced by parkin deficiency in drosophila hearts and its containment by suppressing mitofusin. In: Circulation Research. 2014 ; Vol. 114, No. 2. pp. 257-265.
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