Mitochondria-associated yeast mRNAs and the biogenesis of molecular complexes

M. Garcia, X. Darzacq, T. Delaveau, L. Jourdren, Robert H. Singer, Claude Jacq

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The coherence of mitochondrial biogenesis relies on spatiotemporally coordinated associations of 800-1000 proteins mostly encoded in the nuclear genome. We report the development of new quantitative analyses to assess the role of local protein translation in the construction of molecular complexes. We used real-time PCR to determine the cellular location of 112 mRNAs involved in seven mitochondrial complexes. Five typical cases were examined by an improved FISH protocol. The proteins produced in the vicinity of mitochondria (MLR proteins) were, almost exclusively, of prokaryotic origin and are key elements of the core construction of the molecular complexes; the accessory proteins were translated on free cytoplasmic polysomes. These two classes of proteins correspond, at least as far as intermembrane space (IMS) proteins are concerned, to two different import pathways. Import of MLR proteins involves both TOM and TIM23 complexes whereas non-MLR proteins only interact with the TOM complex. Site-specific translation loci, both outside and inside mitochondria, may coordinate the construction of molecular complexes composed of both nuclearly and mitochondrially encoded subunits.

Original languageEnglish (US)
Pages (from-to)362-368
Number of pages7
JournalMolecular Biology of the Cell
Volume18
Issue number2
DOIs
StatePublished - Feb 2007

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Mitochondria
Yeasts
Messenger RNA
Proteins
Polyribosomes
Protein Biosynthesis
Organelle Biogenesis
Real-Time Polymerase Chain Reaction
Genome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Mitochondria-associated yeast mRNAs and the biogenesis of molecular complexes. / Garcia, M.; Darzacq, X.; Delaveau, T.; Jourdren, L.; Singer, Robert H.; Jacq, Claude.

In: Molecular Biology of the Cell, Vol. 18, No. 2, 02.2007, p. 362-368.

Research output: Contribution to journalArticle

Garcia, M. ; Darzacq, X. ; Delaveau, T. ; Jourdren, L. ; Singer, Robert H. ; Jacq, Claude. / Mitochondria-associated yeast mRNAs and the biogenesis of molecular complexes. In: Molecular Biology of the Cell. 2007 ; Vol. 18, No. 2. pp. 362-368.
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