Mir-24 targets the transmembrane glycoprotein neuropilin-1 in human brain microvascular endothelial cells

Pasquale Mone, Jessica Gambardella, Xujun Wang, Stanislovas S. Jankauskas, Alessandro Matarese, Gaetano Santulli

Research output: Contribution to journalArticlepeer-review

Abstract

Neuropilin-1 is a transmembrane glycoprotein that has been implicated in several processes including angiogenesis and immunity. Recent evidence has also shown that it is implied in the cellular internalization of the severe acute respiratory syndrome coronavirus (SARS-CoV-2), which causes the coronavirus disease 2019 (COVID-19). We hypothesized that specific microRNAs can target Neuropilin-1. By combining bioinformatic and functional approaches, we identified miR-24 as a regulator of Neuropilin-1 transcription. Since Neuropilin-1 has been shown to play a key role in the endothelium-mediated regulation of the blood-brain barrier, we validated miR-24 as a functional modulator of Neuropilin-1 in human brain microvascular endothelial cells (hBMECs), which are the most suitable cell line for an in vitro blood–brain barrier model.

Original languageEnglish (US)
Article number9
Pages (from-to)1-10
Number of pages10
JournalNon-coding RNA
Volume7
Issue number1
DOIs
StatePublished - Mar 2021

Keywords

  • ACE2
  • Akt
  • Blood–brain barrier
  • Brain
  • CD304
  • COVID-19
  • Coronavirus
  • Endothelium
  • Epigenetics
  • HBMEC
  • MiR-24-3p
  • MicroRNA
  • NRP1
  • Neurology
  • Non-coding RNA
  • SARS-CoV-2
  • VEGF
  • VEGF165R
  • Vascular permeability

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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