Minireview: Neuropeptidomics to study peptide processing in animal models of obesity

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Neuropeptidomics is the analysis of the neuropeptides present in a tissue extract. Most neuropeptidomic studies use mass spectrometry to detect and identify the peptides, which provides information on the precise posttranslationally modified form of each peptide. Quantitative peptidomics uses isotopic labels to compare the levels of peptides in extracts from two different samples. This technique is ideal for examining neuropeptide levels in a variety of systems and is especially suited for studies of mice lacking peptide-processing enzymes. This review is focused on the neuropeptidomics technique and its application to the analysis of mice with a mutation that inactivates carboxypeptidase E, a critical enzyme in the biosynthesis of many neuroendocrine peptides. Mice without carboxypeptidase E activity are overweight, and a key question is the identification of the peptide or peptides responsible. The quantitative peptidomics approach has provided some insights toward the answer to this question.

Original languageEnglish (US)
Pages (from-to)4185-4190
Number of pages6
JournalEndocrinology
Volume148
Issue number9
DOIs
StatePublished - Sep 2007

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Animal Models
Obesity
Peptides
Carboxypeptidase H
Neuropeptides
Tissue Extracts
Enzymes
Mass Spectrometry
Mutation

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Minireview : Neuropeptidomics to study peptide processing in animal models of obesity. / Fricker, Lloyd D.

In: Endocrinology, Vol. 148, No. 9, 09.2007, p. 4185-4190.

Research output: Contribution to journalArticle

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