Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow

Heiko Blaser, Michal Reichman-Fried, Irinka Castanon, Karin Dumstrei, Florence L Marlow, Koichi Kawakami, Lilianna Solnica-Krezel, Carl Philipp Heisenberg, Erez Raz

Research output: Contribution to journalArticle

239 Citations (Scopus)

Abstract

The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.

Original languageEnglish (US)
Pages (from-to)613-627
Number of pages15
JournalDevelopmental Cell
Volume11
Issue number5
DOIs
StatePublished - Nov 2006
Externally publishedYes

Fingerprint

Zebrafish
Myosins
Germ Cells
Cell Movement
pioglitazone
Cells
Blister
Chemical activation
CXCR4 Receptors
Calcium
Cell membranes
Chemokines
Cytoplasm
Cell Membrane
Ligands

Keywords

  • CELLBIO
  • DEVBIO
  • STEMCELL

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Blaser, H., Reichman-Fried, M., Castanon, I., Dumstrei, K., Marlow, FL., Kawakami, K., ... Raz, E. (2006). Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow. Developmental Cell, 11(5), 613-627. https://doi.org/10.1016/j.devcel.2006.09.023

Migration of Zebrafish Primordial Germ Cells : A Role for Myosin Contraction and Cytoplasmic Flow. / Blaser, Heiko; Reichman-Fried, Michal; Castanon, Irinka; Dumstrei, Karin; Marlow, Florence L; Kawakami, Koichi; Solnica-Krezel, Lilianna; Heisenberg, Carl Philipp; Raz, Erez.

In: Developmental Cell, Vol. 11, No. 5, 11.2006, p. 613-627.

Research output: Contribution to journalArticle

Blaser, H, Reichman-Fried, M, Castanon, I, Dumstrei, K, Marlow, FL, Kawakami, K, Solnica-Krezel, L, Heisenberg, CP & Raz, E 2006, 'Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow', Developmental Cell, vol. 11, no. 5, pp. 613-627. https://doi.org/10.1016/j.devcel.2006.09.023
Blaser H, Reichman-Fried M, Castanon I, Dumstrei K, Marlow FL, Kawakami K et al. Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow. Developmental Cell. 2006 Nov;11(5):613-627. https://doi.org/10.1016/j.devcel.2006.09.023
Blaser, Heiko ; Reichman-Fried, Michal ; Castanon, Irinka ; Dumstrei, Karin ; Marlow, Florence L ; Kawakami, Koichi ; Solnica-Krezel, Lilianna ; Heisenberg, Carl Philipp ; Raz, Erez. / Migration of Zebrafish Primordial Germ Cells : A Role for Myosin Contraction and Cytoplasmic Flow. In: Developmental Cell. 2006 ; Vol. 11, No. 5. pp. 613-627.
@article{8005a69531b94312a6d369eadda7e2d2,
title = "Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow",
abstract = "The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.",
keywords = "CELLBIO, DEVBIO, STEMCELL",
author = "Heiko Blaser and Michal Reichman-Fried and Irinka Castanon and Karin Dumstrei and Florence L Marlow and Koichi Kawakami and Lilianna Solnica-Krezel and Heisenberg, {Carl Philipp} and Erez Raz",
year = "2006",
month = "11",
doi = "10.1016/j.devcel.2006.09.023",
language = "English (US)",
volume = "11",
pages = "613--627",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "5",

}

TY - JOUR

T1 - Migration of Zebrafish Primordial Germ Cells

T2 - A Role for Myosin Contraction and Cytoplasmic Flow

AU - Blaser, Heiko

AU - Reichman-Fried, Michal

AU - Castanon, Irinka

AU - Dumstrei, Karin

AU - Marlow, Florence L

AU - Kawakami, Koichi

AU - Solnica-Krezel, Lilianna

AU - Heisenberg, Carl Philipp

AU - Raz, Erez

PY - 2006/11

Y1 - 2006/11

N2 - The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.

AB - The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.

KW - CELLBIO

KW - DEVBIO

KW - STEMCELL

UR - http://www.scopus.com/inward/record.url?scp=33750491282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750491282&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2006.09.023

DO - 10.1016/j.devcel.2006.09.023

M3 - Article

C2 - 17084355

AN - SCOPUS:33750491282

VL - 11

SP - 613

EP - 627

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 5

ER -