Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow

Heiko Blaser; Michal Reichman-Fried; Irinka Castanon; Karin Dumstrei; Florence L L. Marlow; Koichi Kawakami; Lilianna Solnica-Krezel; Carl Philipp Heisenberg; Erez Raz

doi:10.1016/j.devcel.2006.09.023

Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow

Heiko Blaser, Michal Reichman-Fried, Irinka Castanon, Karin Dumstrei, Florence L L. Marlow, Koichi Kawakami, Lilianna Solnica-Krezel, Carl Philipp Heisenberg, Erez Raz

Research output: Contribution to journal › Article › peer-review

284 Scopus citations

Abstract

The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.

Original language	English (US)
Pages (from-to)	613-627
Number of pages	15
Journal	Developmental cell
Volume	11
Issue number	5
DOIs	https://doi.org/10.1016/j.devcel.2006.09.023
State	Published - Nov 2006
Externally published	Yes

Keywords

CELLBIO
DEVBIO
STEMCELL

ASJC Scopus subject areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Developmental Biology
Cell Biology

Access to Document

10.1016/j.devcel.2006.09.023

Cite this

@article{8005a69531b94312a6d369eadda7e2d2,

title = "Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow",

abstract = "The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.",

keywords = "CELLBIO, DEVBIO, STEMCELL",

author = "Heiko Blaser and Michal Reichman-Fried and Irinka Castanon and Karin Dumstrei and Marlow, {Florence L L.} and Koichi Kawakami and Lilianna Solnica-Krezel and Heisenberg, {Carl Philipp} and Erez Raz",

note = "Funding Information: We thank Rex Chisholm, Steve Farber, Adi Kimchi, Masamitsu Iino, Klemens Rottner, Heikki Rauvala, and Marcus Fechheimer for kindly providing us with DNA constructs and Helen Le-Cordier and Julia D{\"o}rries for technical assistance. We are grateful to Elena Kardash for helpful discussions and to Sonia Minina and Ewa Paluch for critical reading of the manuscript. This work was supported by funds from the Max Planck Society and grants from the Deutsche Forschungsgemeinschaft to E.R. and to C.-P.H. H.B. was supported by Roche Research Foundation, I.C. by the Humboldt Foundation, K.D. by a European Molecular Biology Organization long-term fellowship, and L.S.K. by National Institutes of Health grants GM55101 and GM 77770. ",

year = "2006",

month = nov,

doi = "10.1016/j.devcel.2006.09.023",

language = "English (US)",

volume = "11",

pages = "613--627",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Cell Press",

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TY - JOUR

T1 - Migration of Zebrafish Primordial Germ Cells

T2 - A Role for Myosin Contraction and Cytoplasmic Flow

AU - Blaser, Heiko

AU - Reichman-Fried, Michal

AU - Castanon, Irinka

AU - Dumstrei, Karin

AU - Marlow, Florence L L.

AU - Kawakami, Koichi

AU - Solnica-Krezel, Lilianna

AU - Heisenberg, Carl Philipp

AU - Raz, Erez

N1 - Funding Information: We thank Rex Chisholm, Steve Farber, Adi Kimchi, Masamitsu Iino, Klemens Rottner, Heikki Rauvala, and Marcus Fechheimer for kindly providing us with DNA constructs and Helen Le-Cordier and Julia Dörries for technical assistance. We are grateful to Elena Kardash for helpful discussions and to Sonia Minina and Ewa Paluch for critical reading of the manuscript. This work was supported by funds from the Max Planck Society and grants from the Deutsche Forschungsgemeinschaft to E.R. and to C.-P.H. H.B. was supported by Roche Research Foundation, I.C. by the Humboldt Foundation, K.D. by a European Molecular Biology Organization long-term fellowship, and L.S.K. by National Institutes of Health grants GM55101 and GM 77770.

PY - 2006/11

Y1 - 2006/11

N2 - The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.

AB - The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.

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KW - DEVBIO

KW - STEMCELL

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Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow

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