Microtubule asymmetry during neutrophil polarization and migration

Robert J. Eddy, Lynda M. Pierini, Frederick R. Maxfield

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The development of cell polarity in response to chemoattractant stimulation in human polymorphonuclear neutrophils (PMNs) is characterized by the rapid conversion from round to polarized morphology with a leading lamellipod at the front and a uropod at the rear. During PMN polarization, the microtubule (MT) array undergoes a dramatic reorientation toward the uropod that is maintained during motility and does not require large-scale MT disassembly or cell adhesion to the substratum. MTs are excluded from the leading lamella during polarization and motility, but treatment with a myosin light chain kinase inhibitor (ML-7) or the actin-disrupting drug cytochalasin D causes an expansion of the MT array and penetration of MTs into the lamellipod. Depolymerization of the MT array before stimulation caused 10% of the cells to lose their polarity by extending two opposing lateral lamellipodia. These multipolar cells showed altered localization of a leading lamella-specific marker, talin, and a uropod-specific marker, CD44. In summary, these results indicate that F-actin- and myosin II-dependent forces lead to the development and maintenance of MT asymmetry that may act to reinforce cell polarity during PMN migration.

Original languageEnglish (US)
Pages (from-to)4470-4483
Number of pages14
JournalMolecular Biology of the Cell
Volume13
Issue number12
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

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Microtubules
Neutrophils
Cell Polarity
Actins
Talin
Myosin Type II
Myosin-Light-Chain Kinase
Cytochalasin D
Pseudopodia
Chemotactic Factors
Cell Adhesion
Maintenance
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Microtubule asymmetry during neutrophil polarization and migration. / Eddy, Robert J.; Pierini, Lynda M.; Maxfield, Frederick R.

In: Molecular Biology of the Cell, Vol. 13, No. 12, 01.12.2002, p. 4470-4483.

Research output: Contribution to journalArticle

Eddy, Robert J. ; Pierini, Lynda M. ; Maxfield, Frederick R. / Microtubule asymmetry during neutrophil polarization and migration. In: Molecular Biology of the Cell. 2002 ; Vol. 13, No. 12. pp. 4470-4483.
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