Abstract
Tryptic peptides and cyanogen bromide fragments of dopamine beta-monooxygenase (DBH) were prepared and separated on C-8 reverse phase columns by high pressure liquid chromatography. Absorbance profiles at both 220 nm and 280 nm were monitored so that peptides with aromatic residues could be isolated. These peptides were subjected to automated Edmann degradation with a gas phase microsequencer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 289-292 |
| Number of pages | 4 |
| Journal | Journal of Neuroscience Research |
| Volume | 18 |
| Issue number | 2 |
| State | Published - 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Microsequencing of dopamine beta-monooxygenase. / McCafferty, B.; Angeletti, R. H.
In: Journal of Neuroscience Research, Vol. 18, No. 2, 1987, p. 289-292.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microsequencing of dopamine beta-monooxygenase
AU - McCafferty, B.
AU - Angeletti, R. H.
PY - 1987
Y1 - 1987
N2 - Tryptic peptides and cyanogen bromide fragments of dopamine beta-monooxygenase (DBH) were prepared and separated on C-8 reverse phase columns by high pressure liquid chromatography. Absorbance profiles at both 220 nm and 280 nm were monitored so that peptides with aromatic residues could be isolated. These peptides were subjected to automated Edmann degradation with a gas phase microsequencer.
AB - Tryptic peptides and cyanogen bromide fragments of dopamine beta-monooxygenase (DBH) were prepared and separated on C-8 reverse phase columns by high pressure liquid chromatography. Absorbance profiles at both 220 nm and 280 nm were monitored so that peptides with aromatic residues could be isolated. These peptides were subjected to automated Edmann degradation with a gas phase microsequencer.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023574442&partnerID=8YFLogxK
M3 - Article
C2 - 3694711
AN - SCOPUS:0023574442
VL - 18
SP - 289
EP - 292
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 2
ER -