MicroRNA expression aids the preoperative diagnosis of pancreatic ductal adenocarcinoma

Nicole C. Panarelli, Yao Tseng Chen, Xi K. Zhou, Naoki Kitabayashi, Rhonda K. Yantiss

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Objectives: This study aimed to evaluate microRNA (miRNA) expression in pancreatic resection specimens and fine needle aspiration biopsies and determine which, if any, miRNAs aid the distinction between benign and malignant pancreatic tumors in limited cytology material. Methods: Resection specimens containing adenocarcinoma (n = 17), intraductal papillary mucinous neoplasms (n = 11), and nonneoplastic tissues (n = 15) were evaluated for miR-21, miR-221, miR-100, miR-155, and miR-181b expression by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and a subset of carcinomas and intraductal papillary mucinous neoplasms was analyzed with miRNA microarrays. Cellblocks containing carcinoma (n = 26) or benign pancreatic lesions (n = 11) from fine needle aspiration biopsies were subjected to qRT-PCR for miR-21, miR-221, miR-181b, miR-196a, and miR-217. Results: Carcinomas showed higher expression of miR-21, miR-221, miR-155, miR-100, and miR-181b than benign lesions by qRT-PCR, and overexpression of miR-21, miR-221, and miR-181b was confirmed by microarray analysis. Cellblocks containing carcinoma showed higher expression of miR-21, miR-221, and miR-196a than those from benign lesions (P <0.001, P = 0.009, and P <0.001, respectively). Conclusions: Pancreatic ductal adenocarcinomas show differential expression of miRNAs compared to benign pancreatic lesions. A select panel of miRNAs aids the distinction between pancreatic lesions in cytology specimens.

Original languageEnglish (US)
Pages (from-to)685-690
Number of pages6
JournalPancreas
Volume41
Issue number5
DOIs
StatePublished - Jul 2012
Externally publishedYes

Fingerprint

MicroRNAs
Adenocarcinoma
Reverse Transcriptase Polymerase Chain Reaction
Fine Needle Biopsy
Carcinoma
Cell Biology
Neoplasms
Carcinoma, Intraductal, Noninfiltrating
Papillary Carcinoma
Microarray Analysis

Keywords

  • Cytology
  • Fine needle aspiration
  • Intraductal papillary mucinous neoplasm
  • Molecular
  • Pancreas

ASJC Scopus subject areas

  • Hepatology
  • Internal Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

MicroRNA expression aids the preoperative diagnosis of pancreatic ductal adenocarcinoma. / Panarelli, Nicole C.; Chen, Yao Tseng; Zhou, Xi K.; Kitabayashi, Naoki; Yantiss, Rhonda K.

In: Pancreas, Vol. 41, No. 5, 07.2012, p. 685-690.

Research output: Contribution to journalArticle

Panarelli, Nicole C. ; Chen, Yao Tseng ; Zhou, Xi K. ; Kitabayashi, Naoki ; Yantiss, Rhonda K. / MicroRNA expression aids the preoperative diagnosis of pancreatic ductal adenocarcinoma. In: Pancreas. 2012 ; Vol. 41, No. 5. pp. 685-690.
@article{e905fc76009f4903b0be92d7b41eac8f,
title = "MicroRNA expression aids the preoperative diagnosis of pancreatic ductal adenocarcinoma",
abstract = "Objectives: This study aimed to evaluate microRNA (miRNA) expression in pancreatic resection specimens and fine needle aspiration biopsies and determine which, if any, miRNAs aid the distinction between benign and malignant pancreatic tumors in limited cytology material. Methods: Resection specimens containing adenocarcinoma (n = 17), intraductal papillary mucinous neoplasms (n = 11), and nonneoplastic tissues (n = 15) were evaluated for miR-21, miR-221, miR-100, miR-155, and miR-181b expression by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and a subset of carcinomas and intraductal papillary mucinous neoplasms was analyzed with miRNA microarrays. Cellblocks containing carcinoma (n = 26) or benign pancreatic lesions (n = 11) from fine needle aspiration biopsies were subjected to qRT-PCR for miR-21, miR-221, miR-181b, miR-196a, and miR-217. Results: Carcinomas showed higher expression of miR-21, miR-221, miR-155, miR-100, and miR-181b than benign lesions by qRT-PCR, and overexpression of miR-21, miR-221, and miR-181b was confirmed by microarray analysis. Cellblocks containing carcinoma showed higher expression of miR-21, miR-221, and miR-196a than those from benign lesions (P <0.001, P = 0.009, and P <0.001, respectively). Conclusions: Pancreatic ductal adenocarcinomas show differential expression of miRNAs compared to benign pancreatic lesions. A select panel of miRNAs aids the distinction between pancreatic lesions in cytology specimens.",
keywords = "Cytology, Fine needle aspiration, Intraductal papillary mucinous neoplasm, Molecular, Pancreas",
author = "Panarelli, {Nicole C.} and Chen, {Yao Tseng} and Zhou, {Xi K.} and Naoki Kitabayashi and Yantiss, {Rhonda K.}",
year = "2012",
month = "7",
doi = "10.1097/MPA.0b013e318243a905",
language = "English (US)",
volume = "41",
pages = "685--690",
journal = "Pancreas",
issn = "0885-3177",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - MicroRNA expression aids the preoperative diagnosis of pancreatic ductal adenocarcinoma

AU - Panarelli, Nicole C.

AU - Chen, Yao Tseng

AU - Zhou, Xi K.

AU - Kitabayashi, Naoki

AU - Yantiss, Rhonda K.

PY - 2012/7

Y1 - 2012/7

N2 - Objectives: This study aimed to evaluate microRNA (miRNA) expression in pancreatic resection specimens and fine needle aspiration biopsies and determine which, if any, miRNAs aid the distinction between benign and malignant pancreatic tumors in limited cytology material. Methods: Resection specimens containing adenocarcinoma (n = 17), intraductal papillary mucinous neoplasms (n = 11), and nonneoplastic tissues (n = 15) were evaluated for miR-21, miR-221, miR-100, miR-155, and miR-181b expression by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and a subset of carcinomas and intraductal papillary mucinous neoplasms was analyzed with miRNA microarrays. Cellblocks containing carcinoma (n = 26) or benign pancreatic lesions (n = 11) from fine needle aspiration biopsies were subjected to qRT-PCR for miR-21, miR-221, miR-181b, miR-196a, and miR-217. Results: Carcinomas showed higher expression of miR-21, miR-221, miR-155, miR-100, and miR-181b than benign lesions by qRT-PCR, and overexpression of miR-21, miR-221, and miR-181b was confirmed by microarray analysis. Cellblocks containing carcinoma showed higher expression of miR-21, miR-221, and miR-196a than those from benign lesions (P <0.001, P = 0.009, and P <0.001, respectively). Conclusions: Pancreatic ductal adenocarcinomas show differential expression of miRNAs compared to benign pancreatic lesions. A select panel of miRNAs aids the distinction between pancreatic lesions in cytology specimens.

AB - Objectives: This study aimed to evaluate microRNA (miRNA) expression in pancreatic resection specimens and fine needle aspiration biopsies and determine which, if any, miRNAs aid the distinction between benign and malignant pancreatic tumors in limited cytology material. Methods: Resection specimens containing adenocarcinoma (n = 17), intraductal papillary mucinous neoplasms (n = 11), and nonneoplastic tissues (n = 15) were evaluated for miR-21, miR-221, miR-100, miR-155, and miR-181b expression by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and a subset of carcinomas and intraductal papillary mucinous neoplasms was analyzed with miRNA microarrays. Cellblocks containing carcinoma (n = 26) or benign pancreatic lesions (n = 11) from fine needle aspiration biopsies were subjected to qRT-PCR for miR-21, miR-221, miR-181b, miR-196a, and miR-217. Results: Carcinomas showed higher expression of miR-21, miR-221, miR-155, miR-100, and miR-181b than benign lesions by qRT-PCR, and overexpression of miR-21, miR-221, and miR-181b was confirmed by microarray analysis. Cellblocks containing carcinoma showed higher expression of miR-21, miR-221, and miR-196a than those from benign lesions (P <0.001, P = 0.009, and P <0.001, respectively). Conclusions: Pancreatic ductal adenocarcinomas show differential expression of miRNAs compared to benign pancreatic lesions. A select panel of miRNAs aids the distinction between pancreatic lesions in cytology specimens.

KW - Cytology

KW - Fine needle aspiration

KW - Intraductal papillary mucinous neoplasm

KW - Molecular

KW - Pancreas

UR - http://www.scopus.com/inward/record.url?scp=84863727479&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863727479&partnerID=8YFLogxK

U2 - 10.1097/MPA.0b013e318243a905

DO - 10.1097/MPA.0b013e318243a905

M3 - Article

C2 - 22466166

AN - SCOPUS:84863727479

VL - 41

SP - 685

EP - 690

JO - Pancreas

JF - Pancreas

SN - 0885-3177

IS - 5

ER -