MicroRNA-17 post-transcriptionally regulates polycystic kidney disease-2 gene and promotes cell proliferation

Huan Sun, Qing Wei Li, Xio Yan Lv, Jian Zhong Ai, Qiu Tan Yang, Jing Jing Duan, Guo Hui Bian, Yan Xiao, Yi Dong Wang, Zheng Zhang, Yu Hang Liu, Rui Zhi Tan, Yang Yang, Yu Quan Wei, Qin Zhou

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

To identify the possible microRNAs (miRNAs) which target the polycystic kidney disease-2 gene (PKD2), and clarify effects of the miRNAs on PKD2. We preliminarily used bioinformatics to analyze 3′UTR (3′untranslated regions) of PKD1 and PKD2 in order to predict the potential microRNAs targeted on them. Subsequently, the stable cell lines with overexpression of microRNA-17 (miR-17) were screened, and luciferase assay combined with the mutation 3′UTR of PKD2 were performed to verify PKD2 is the target of miR-17. Moreover, RT-PCR and Western Blotting were used to determine the post-transcriptionally regulation of PKD2 by miR-17. Finally, MTT cell assays allied with PKD2 rescued strategy were employed to evaluate cell proliferation effects. Our study firstly found that the 3′UTR of PKD2 was more conservation than that of PKD1, and microRNA-17 directly targets the 3′UTR of PKD2 and post-transcriptionally repress the expression of PKD2. Moreover, our findings also demonstrated that overexpression of miR-17 may promote cell proliferation via post-transcriptionally repression of PKD2 in HEK 293T. This suggested that microRNA might be a novel mechanism for cystogenesis as well as a potential therapeutic target for the cell proliferation of autosomal dominant polycystic kidney disease (ADPKD).

Original languageEnglish (US)
Pages (from-to)2951-2958
Number of pages8
JournalMolecular Biology Reports
Volume37
Issue number6
DOIs
StatePublished - Jul 1 2010

Keywords

  • Cell proliferation
  • PKD2
  • Polycystin-2
  • miR-17
  • microRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Sun, H., Li, Q. W., Lv, X. Y., Ai, J. Z., Yang, Q. T., Duan, J. J., Bian, G. H., Xiao, Y., Wang, Y. D., Zhang, Z., Liu, Y. H., Tan, R. Z., Yang, Y., Wei, Y. Q., & Zhou, Q. (2010). MicroRNA-17 post-transcriptionally regulates polycystic kidney disease-2 gene and promotes cell proliferation. Molecular Biology Reports, 37(6), 2951-2958. https://doi.org/10.1007/s11033-009-9861-3