Microkeratome versus femtosecond laser predissection of corneal grafts for anterior and posterior lamellar keratoplasty

Olan Suwan-Apichon, Johann M G Reyes, Neil B. Griffin, Jerry Barker, Patrick Gore, Roy S. Chuck

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

PURPOSE: To compare 2 different techniques for predissection of human anterior and posterior lamellar corneal grafts for eye bank storage. METHODS: A mechanical microkeratome (group 1, N = 5) and a femtosecond laser (group 2, N = 5) were used to dissect intended 350-μm-deep lamellar planes in deepithelialized donor corneas mounted on an artificial anterior chamber. These corneas were replaced in Optisol GS at 4°C postoperatively and examined 2 days later to simulate a clinical scenario. Ultrasonic pachymetry of corneal lamellar sections was measured before and after separation of the lamellar grafts. Group 1 sections were separated by the mechanical microkeratome, whereas group 2 sections were manually separated 2 days after laser dissection. Endothelial cell viability was evaluated in posterior grafts. RESULTS: Total corneal thicknesses immediately before dissection were 559 ± 61 (group 1) and 578 ± 79 μm (group 2; P = 0.46). Immediate postdissection anterior and posterior graft thicknesses were 361 ± 68 and 203 ± 74 μm (group 1), respectively. Achieved anterior and posterior graft thicknesses 2 days later were 282 ± 44 and 413 ± 35 μm (group 1) and 324 ± 112 and 397 ± 51 μm (group 2), respectively. Percentage of devitalized endothelial cells were 3.4% ± 1.6% (group 1) and 1.6% ± 1.2% (group 2; P = 0.35). CONCLUSIONS: Centralized predissection by both techniques, cold storage, and shipping by airmail results in viable grafts without significant endothelial cell loss 2 days later.

Original languageEnglish (US)
Pages (from-to)966-968
Number of pages3
JournalCornea
Volume25
Issue number8
DOIs
StatePublished - Nov 2006
Externally publishedYes

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Corneal Transplantation
Lasers
Transplants
Endothelial Cells
Cornea
Dissection
Eye Banks
Corneal Pachymetry
Anterior Chamber
Ultrasonics
Cell Survival

Keywords

  • Anterior
  • Cornea
  • Endothelium
  • Femtosecond
  • Lamellar
  • Laser
  • Microkeratome
  • Posterior
  • Transplant

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Microkeratome versus femtosecond laser predissection of corneal grafts for anterior and posterior lamellar keratoplasty. / Suwan-Apichon, Olan; Reyes, Johann M G; Griffin, Neil B.; Barker, Jerry; Gore, Patrick; Chuck, Roy S.

In: Cornea, Vol. 25, No. 8, 11.2006, p. 966-968.

Research output: Contribution to journalArticle

Suwan-Apichon, Olan ; Reyes, Johann M G ; Griffin, Neil B. ; Barker, Jerry ; Gore, Patrick ; Chuck, Roy S. / Microkeratome versus femtosecond laser predissection of corneal grafts for anterior and posterior lamellar keratoplasty. In: Cornea. 2006 ; Vol. 25, No. 8. pp. 966-968.
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AU - Suwan-Apichon, Olan

AU - Reyes, Johann M G

AU - Griffin, Neil B.

AU - Barker, Jerry

AU - Gore, Patrick

AU - Chuck, Roy S.

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N2 - PURPOSE: To compare 2 different techniques for predissection of human anterior and posterior lamellar corneal grafts for eye bank storage. METHODS: A mechanical microkeratome (group 1, N = 5) and a femtosecond laser (group 2, N = 5) were used to dissect intended 350-μm-deep lamellar planes in deepithelialized donor corneas mounted on an artificial anterior chamber. These corneas were replaced in Optisol GS at 4°C postoperatively and examined 2 days later to simulate a clinical scenario. Ultrasonic pachymetry of corneal lamellar sections was measured before and after separation of the lamellar grafts. Group 1 sections were separated by the mechanical microkeratome, whereas group 2 sections were manually separated 2 days after laser dissection. Endothelial cell viability was evaluated in posterior grafts. RESULTS: Total corneal thicknesses immediately before dissection were 559 ± 61 (group 1) and 578 ± 79 μm (group 2; P = 0.46). Immediate postdissection anterior and posterior graft thicknesses were 361 ± 68 and 203 ± 74 μm (group 1), respectively. Achieved anterior and posterior graft thicknesses 2 days later were 282 ± 44 and 413 ± 35 μm (group 1) and 324 ± 112 and 397 ± 51 μm (group 2), respectively. Percentage of devitalized endothelial cells were 3.4% ± 1.6% (group 1) and 1.6% ± 1.2% (group 2; P = 0.35). CONCLUSIONS: Centralized predissection by both techniques, cold storage, and shipping by airmail results in viable grafts without significant endothelial cell loss 2 days later.

AB - PURPOSE: To compare 2 different techniques for predissection of human anterior and posterior lamellar corneal grafts for eye bank storage. METHODS: A mechanical microkeratome (group 1, N = 5) and a femtosecond laser (group 2, N = 5) were used to dissect intended 350-μm-deep lamellar planes in deepithelialized donor corneas mounted on an artificial anterior chamber. These corneas were replaced in Optisol GS at 4°C postoperatively and examined 2 days later to simulate a clinical scenario. Ultrasonic pachymetry of corneal lamellar sections was measured before and after separation of the lamellar grafts. Group 1 sections were separated by the mechanical microkeratome, whereas group 2 sections were manually separated 2 days after laser dissection. Endothelial cell viability was evaluated in posterior grafts. RESULTS: Total corneal thicknesses immediately before dissection were 559 ± 61 (group 1) and 578 ± 79 μm (group 2; P = 0.46). Immediate postdissection anterior and posterior graft thicknesses were 361 ± 68 and 203 ± 74 μm (group 1), respectively. Achieved anterior and posterior graft thicknesses 2 days later were 282 ± 44 and 413 ± 35 μm (group 1) and 324 ± 112 and 397 ± 51 μm (group 2), respectively. Percentage of devitalized endothelial cells were 3.4% ± 1.6% (group 1) and 1.6% ± 1.2% (group 2; P = 0.35). CONCLUSIONS: Centralized predissection by both techniques, cold storage, and shipping by airmail results in viable grafts without significant endothelial cell loss 2 days later.

KW - Anterior

KW - Cornea

KW - Endothelium

KW - Femtosecond

KW - Lamellar

KW - Laser

KW - Microkeratome

KW - Posterior

KW - Transplant

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