Microkeratome preparation of lamellar corneal grafts

Olan Suwan-Apichon, Johann M G Reyes, Neil B. Griffin, Jerry Barker, Patrick Gore, Roy S. Chuck

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

PURPOSE. To evaluate the thickness and viability of microkeratome-prepared lamellar corneal grafts in cold storage. METHODS. Ten human corneas were sectioned with a mechanical microkeratome with a 350-μm depth head and stored in Optisol GS at 4°C for 2 days to simulate an eye bank scenario. Central corneal thickness before and after mechanical microkeratome sectioning was measured by ultrasonic pachymetry. Endothelial cell viability was evaluated by trypan blue and alizarin red staining. RESULTS. Total corneal thickness immediately before microkeratome dissection was 562 ± 51 μm. Anterior and posterior graft thicknesses were 296 ± 111 μm and 270 ± 74 μm, respectively, immediately after dissection, and 282 ± 38 μm and 429 ± 31 μm, respectively, 2 days after storage. There was significant swelling in the posterior (P=0.005) but not the anterior grafts (P=0.386). The percentage of devitalized endothelial cells was 3.0% ± 1.2%. CONCLUSIONS. Corneal lamellar grafts may possibly be precut in a centralized facility and stored cold before further distribution.

Original languageEnglish (US)
Pages (from-to)248-249
Number of pages2
JournalEye and Contact Lens
Volume32
Issue number5
DOIs
StatePublished - Sep 2006
Externally publishedYes

Fingerprint

Transplants
Dissection
Endothelial Cells
Eye Banks
Trypan Blue
Ultrasonics
Cornea
Cell Survival
Head
Staining and Labeling
alizarin
trypan red
Optisol

Keywords

  • Corneal endothelial cell
  • Corneal thickness
  • Donor cornea preparation
  • Lamellar corneal graft
  • Microkeratome

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Suwan-Apichon, O., Reyes, J. M. G., Griffin, N. B., Barker, J., Gore, P., & Chuck, R. S. (2006). Microkeratome preparation of lamellar corneal grafts. Eye and Contact Lens, 32(5), 248-249. https://doi.org/10.1097/01.icl.0000219364.21999.e4

Microkeratome preparation of lamellar corneal grafts. / Suwan-Apichon, Olan; Reyes, Johann M G; Griffin, Neil B.; Barker, Jerry; Gore, Patrick; Chuck, Roy S.

In: Eye and Contact Lens, Vol. 32, No. 5, 09.2006, p. 248-249.

Research output: Contribution to journalArticle

Suwan-Apichon, O, Reyes, JMG, Griffin, NB, Barker, J, Gore, P & Chuck, RS 2006, 'Microkeratome preparation of lamellar corneal grafts', Eye and Contact Lens, vol. 32, no. 5, pp. 248-249. https://doi.org/10.1097/01.icl.0000219364.21999.e4
Suwan-Apichon, Olan ; Reyes, Johann M G ; Griffin, Neil B. ; Barker, Jerry ; Gore, Patrick ; Chuck, Roy S. / Microkeratome preparation of lamellar corneal grafts. In: Eye and Contact Lens. 2006 ; Vol. 32, No. 5. pp. 248-249.
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N2 - PURPOSE. To evaluate the thickness and viability of microkeratome-prepared lamellar corneal grafts in cold storage. METHODS. Ten human corneas were sectioned with a mechanical microkeratome with a 350-μm depth head and stored in Optisol GS at 4°C for 2 days to simulate an eye bank scenario. Central corneal thickness before and after mechanical microkeratome sectioning was measured by ultrasonic pachymetry. Endothelial cell viability was evaluated by trypan blue and alizarin red staining. RESULTS. Total corneal thickness immediately before microkeratome dissection was 562 ± 51 μm. Anterior and posterior graft thicknesses were 296 ± 111 μm and 270 ± 74 μm, respectively, immediately after dissection, and 282 ± 38 μm and 429 ± 31 μm, respectively, 2 days after storage. There was significant swelling in the posterior (P=0.005) but not the anterior grafts (P=0.386). The percentage of devitalized endothelial cells was 3.0% ± 1.2%. CONCLUSIONS. Corneal lamellar grafts may possibly be precut in a centralized facility and stored cold before further distribution.

AB - PURPOSE. To evaluate the thickness and viability of microkeratome-prepared lamellar corneal grafts in cold storage. METHODS. Ten human corneas were sectioned with a mechanical microkeratome with a 350-μm depth head and stored in Optisol GS at 4°C for 2 days to simulate an eye bank scenario. Central corneal thickness before and after mechanical microkeratome sectioning was measured by ultrasonic pachymetry. Endothelial cell viability was evaluated by trypan blue and alizarin red staining. RESULTS. Total corneal thickness immediately before microkeratome dissection was 562 ± 51 μm. Anterior and posterior graft thicknesses were 296 ± 111 μm and 270 ± 74 μm, respectively, immediately after dissection, and 282 ± 38 μm and 429 ± 31 μm, respectively, 2 days after storage. There was significant swelling in the posterior (P=0.005) but not the anterior grafts (P=0.386). The percentage of devitalized endothelial cells was 3.0% ± 1.2%. CONCLUSIONS. Corneal lamellar grafts may possibly be precut in a centralized facility and stored cold before further distribution.

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