Microkeratome-based limbal harvester for limbal stem cell transplantation: Preliminary studies

Roy S. Chuck, Ashley Behrens, Peter J. McDonnell

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

PURPOSE: To develop a mechanical device to perform limbal transplantation. This procedure is a valuable surgical technique for management of limbal stem cell deficiency. However, the freehand dissection to obtain donor tissue is laborious and time-consuming. METHODS: A manual microkeratome (LSK One; Moria/Microtech, Doylestown, Pennsylvania) using a redesigned head (200 μm thickness, 16-mm blade) was used to create partial thickness corneoscleral caps from human donor globes. RESULTS: Corneoscleral caps obtained from human donor globes included a ring of approximately 1 mm of perilimbal sclera in contiguity to the cornea. Scanning electron microscopy showed a smooth cut surface with some chatter at the margins. CONCLUSION: This instrument ensures an effective and straightforward method to dissect the area where stem cells have been indirectly localized.

Original languageEnglish (US)
Pages (from-to)377-378
Number of pages2
JournalAmerican journal of ophthalmology
Volume131
Issue number3
DOIs
StatePublished - Mar 13 2001
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology

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