Microkeratome-based limbal harvester for limbal stem cell transplantation: Preliminary studies

Roy S. Chuck, Ashley Behrens, Peter J. McDonnell

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

PURPOSE: To develop a mechanical device to perform limbal transplantation. This procedure is a valuable surgical technique for management of limbal stem cell deficiency. However, the freehand dissection to obtain donor tissue is laborious and time-consuming. METHODS: A manual microkeratome (LSK One; Moria/Microtech, Doylestown, Pennsylvania) using a redesigned head (200 μm thickness, 16-mm blade) was used to create partial thickness corneoscleral caps from human donor globes. RESULTS: Corneoscleral caps obtained from human donor globes included a ring of approximately 1 mm of perilimbal sclera in contiguity to the cornea. Scanning electron microscopy showed a smooth cut surface with some chatter at the margins. CONCLUSION: This instrument ensures an effective and straightforward method to dissect the area where stem cells have been indirectly localized.

Original languageEnglish (US)
Pages (from-to)377-378
Number of pages2
JournalAmerican Journal of Ophthalmology
Volume131
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Stem Cell Transplantation
Stem Cells
Sclera
Electron Scanning Microscopy
Cornea
Dissection
Transplantation
Head
Tissue Donors
Equipment and Supplies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Microkeratome-based limbal harvester for limbal stem cell transplantation : Preliminary studies. / Chuck, Roy S.; Behrens, Ashley; McDonnell, Peter J.

In: American Journal of Ophthalmology, Vol. 131, No. 3, 2001, p. 377-378.

Research output: Contribution to journalArticle

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