Abstract
Independently derived mutants of Chinese hamster ovary cells have been isolated and shown to exhibit a subtle glycosylation defect resulting in the premature termination of certain asparagine-linked carbohydrate moieties. This carbohydrate alteration is akin to the types of structural variation termed microheterogeneity and is thought not to affect the biological activities of glycoproteins that manifest the phenomenon. However, the carbohydrate change expressed by the mutants is stable and heritable, and 1251-lectin-binding studies suggest that it profoundly alters their surface recognition properties. The mutation appears to affect a specific subpopulation of galactose residues in asparagine-linked carbohydrate of the type found associated with the G glycoprotein of vesicular stomatitis virus. The mutant cells also exhibit morphological changes in substratum culture.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 763-769 |
| Number of pages | 7 |
| Journal | Cell |
| Volume | 23 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1981 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Medicine(all)
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Microheterogeneity among carbohydrate structures at the cell surface may be important in recognition phenomena. / Stanley, Pamela; Sudo, Tadashi.
In: Cell, Vol. 23, No. 3, 1981, p. 763-769.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microheterogeneity among carbohydrate structures at the cell surface may be important in recognition phenomena
AU - Stanley, Pamela
AU - Sudo, Tadashi
PY - 1981
Y1 - 1981
N2 - Independently derived mutants of Chinese hamster ovary cells have been isolated and shown to exhibit a subtle glycosylation defect resulting in the premature termination of certain asparagine-linked carbohydrate moieties. This carbohydrate alteration is akin to the types of structural variation termed microheterogeneity and is thought not to affect the biological activities of glycoproteins that manifest the phenomenon. However, the carbohydrate change expressed by the mutants is stable and heritable, and 1251-lectin-binding studies suggest that it profoundly alters their surface recognition properties. The mutation appears to affect a specific subpopulation of galactose residues in asparagine-linked carbohydrate of the type found associated with the G glycoprotein of vesicular stomatitis virus. The mutant cells also exhibit morphological changes in substratum culture.
AB - Independently derived mutants of Chinese hamster ovary cells have been isolated and shown to exhibit a subtle glycosylation defect resulting in the premature termination of certain asparagine-linked carbohydrate moieties. This carbohydrate alteration is akin to the types of structural variation termed microheterogeneity and is thought not to affect the biological activities of glycoproteins that manifest the phenomenon. However, the carbohydrate change expressed by the mutants is stable and heritable, and 1251-lectin-binding studies suggest that it profoundly alters their surface recognition properties. The mutation appears to affect a specific subpopulation of galactose residues in asparagine-linked carbohydrate of the type found associated with the G glycoprotein of vesicular stomatitis virus. The mutant cells also exhibit morphological changes in substratum culture.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019436102&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(81)90440-2
DO - 10.1016/0092-8674(81)90440-2
M3 - Article
VL - 23
SP - 763
EP - 769
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -