Microglia activation and gene expression alteration of neurotrophins in the hippocampus following early-life exposure to E-cigarette aerosols in a murine model

Judith T. Zelikoff, Nancy L. Parmalee, Kevin Corbett, Terry Gordon, Catherine B. Klein, Michael Aschner

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Recent epidemiological data indicate that the popularity of electronic cigarettes (e-cigarettes), and consequently nicotine use, is rising in both adolescent and adult populations. As nicotine is a known developmental neurotoxin, these products present a potential threat for those exposed during early life stages. Despite this, few studies have evaluated the toxicity of e-cigarettes on the developing central nervous system. The goal of this study was to assess neurotoxicity resulting from early-life exposure to electronic cigarette aerosols in an in vivo model. Specifically, studies here focused on neuroparameters related to neuroinflammation and neurotrophins. To accomplish this, pregnant and neonatal C57BL/6 mice were exposed to aerosols produced from classic tobacco flavor e-cigarette cartridges (with [13 mg/ml] and without nicotine) during gestation (~3 weeks) and lactation (~3 weeks) via whole-body inhalation. Exposure to e-cigarette aerosols with and without nicotine caused significant reductions in hippocampal gene expression of Ngfr and Bdnf, as well as in serum levels of cytokines IL-1β, IL-2, and IL-6. Exposure to e-cigarette aerosols without nicotine enhanced expression of Iba-1, a specific marker of microglia, in the cornus ammonis 1 region of the hippocampus. Overall, our novel results indicate that exposure to e-cigarette aerosols, with and without nicotine, poses a considerable risk to the developing central nervous system. Consequently, e-cigarettes should be considered a potential public health threat, especially early in life, requiring further research and policy considerations.

Original languageEnglish (US)
Pages (from-to)276-286
Number of pages11
JournalToxicological Sciences
Volume162
Issue number1
DOIs
StatePublished - Jan 1 2018

Fingerprint

Nerve Growth Factors
Microglia
Aerosols
Gene expression
Tobacco Products
Hippocampus
Chemical activation
Nicotine
Gene Expression
Neurology
Central Nervous System
Cornus
Tobacco
Electronic Cigarettes
Flavors
Neurotoxins
Public health
Interleukin-1
Inbred C57BL Mouse
Lactation

Keywords

  • Astrocytes
  • Developmental neurotoxicology
  • Neurotoxicity
  • Neurotoxicology
  • Neurotransmitter

ASJC Scopus subject areas

  • Toxicology

Cite this

Microglia activation and gene expression alteration of neurotrophins in the hippocampus following early-life exposure to E-cigarette aerosols in a murine model. / Zelikoff, Judith T.; Parmalee, Nancy L.; Corbett, Kevin; Gordon, Terry; Klein, Catherine B.; Aschner, Michael.

In: Toxicological Sciences, Vol. 162, No. 1, 01.01.2018, p. 276-286.

Research output: Contribution to journalArticle

Zelikoff, Judith T. ; Parmalee, Nancy L. ; Corbett, Kevin ; Gordon, Terry ; Klein, Catherine B. ; Aschner, Michael. / Microglia activation and gene expression alteration of neurotrophins in the hippocampus following early-life exposure to E-cigarette aerosols in a murine model. In: Toxicological Sciences. 2018 ; Vol. 162, No. 1. pp. 276-286.
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