Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores

Johanna Rivera; Alfred Morgenstern; Frank Bruchertseifer; John F. Kearney; Charles L. Turnbough; Ekaterina Dadachova; Arturo Casadevall

doi:10.1128/AAC.01266-13

Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores

Johanna Rivera, Alfred Morgenstern, Frank Bruchertseifer, John F. Kearney, Charles L. Turnbough, Ekaterina Dadachova, Arturo Casadevall

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting ²¹³Bi-labeled monoclonal antibody (MAb), EA2-1 (²¹³Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to ²¹³Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 μCi ²¹³Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, ²¹³Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.

Original language	English (US)
Pages (from-to)	1813-1815
Number of pages	3
Journal	Antimicrobial agents and chemotherapy
Volume	58
Issue number	3
DOIs	https://doi.org/10.1128/AAC.01266-13
State	Published - Mar 2014

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AAC.01266-13

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title = "Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores",

abstract = "Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting 213Bi-labeled monoclonal antibody (MAb), EA2-1 (213Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 μCi 213Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, 213Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.",

author = "Johanna Rivera and Alfred Morgenstern and Frank Bruchertseifer and Kearney, {John F.} and Turnbough, {Charles L.} and Ekaterina Dadachova and Arturo Casadevall",

year = "2014",

month = mar,

doi = "10.1128/AAC.01266-13",

language = "English (US)",

volume = "58",

pages = "1813--1815",

journal = "Antimicrobial agents and chemotherapy",

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publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores

AU - Rivera, Johanna

AU - Morgenstern, Alfred

AU - Bruchertseifer, Frank

AU - Kearney, John F.

AU - Turnbough, Charles L.

AU - Dadachova, Ekaterina

AU - Casadevall, Arturo

PY - 2014/3

Y1 - 2014/3

N2 - Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting 213Bi-labeled monoclonal antibody (MAb), EA2-1 (213Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 μCi 213Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, 213Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.

AB - Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting 213Bi-labeled monoclonal antibody (MAb), EA2-1 (213Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 μCi 213Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, 213Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.

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Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores

Abstract

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UN SDGs

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