Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores

Johanna Rivera, Alfred Morgenstern, Frank Bruchertseifer, John F. Kearney, Charles L. Turnbough, Ekaterina Dadachova, Arturo Casadevall

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting 213Bi-labeled monoclonal antibody (MAb), EA2-1 (213Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 μCi 213Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, 213Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.

Original languageEnglish (US)
Pages (from-to)1813-1815
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume58
Issue number3
DOIs
StatePublished - Mar 2014

Fingerprint

Alpha Particles
Bacillus anthracis
Spores
Radioimmunotherapy
Monoclonal Antibodies
Immunodominant Epitopes
Antibody Affinity
Antigens
Infection

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Rivera, J., Morgenstern, A., Bruchertseifer, F., Kearney, J. F., Turnbough, C. L., Dadachova, E., & Casadevall, A. (2014). Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores. Antimicrobial Agents and Chemotherapy, 58(3), 1813-1815. https://doi.org/10.1128/AAC.01266-13

Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores. / Rivera, Johanna; Morgenstern, Alfred; Bruchertseifer, Frank; Kearney, John F.; Turnbough, Charles L.; Dadachova, Ekaterina; Casadevall, Arturo.

In: Antimicrobial Agents and Chemotherapy, Vol. 58, No. 3, 03.2014, p. 1813-1815.

Research output: Contribution to journalArticle

Rivera, J, Morgenstern, A, Bruchertseifer, F, Kearney, JF, Turnbough, CL, Dadachova, E & Casadevall, A 2014, 'Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores', Antimicrobial Agents and Chemotherapy, vol. 58, no. 3, pp. 1813-1815. https://doi.org/10.1128/AAC.01266-13
Rivera, Johanna ; Morgenstern, Alfred ; Bruchertseifer, Frank ; Kearney, John F. ; Turnbough, Charles L. ; Dadachova, Ekaterina ; Casadevall, Arturo. / Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores. In: Antimicrobial Agents and Chemotherapy. 2014 ; Vol. 58, No. 3. pp. 1813-1815.
@article{92a8599cd9044750be11be407373fbd9,
title = "Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores",
abstract = "Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting 213Bi-labeled monoclonal antibody (MAb), EA2-1 (213Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 μCi 213Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, 213Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.",
author = "Johanna Rivera and Alfred Morgenstern and Frank Bruchertseifer and Kearney, {John F.} and Turnbough, {Charles L.} and Ekaterina Dadachova and Arturo Casadevall",
year = "2014",
month = "3",
doi = "10.1128/AAC.01266-13",
language = "English (US)",
volume = "58",
pages = "1813--1815",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores

AU - Rivera, Johanna

AU - Morgenstern, Alfred

AU - Bruchertseifer, Frank

AU - Kearney, John F.

AU - Turnbough, Charles L.

AU - Dadachova, Ekaterina

AU - Casadevall, Arturo

PY - 2014/3

Y1 - 2014/3

N2 - Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting 213Bi-labeled monoclonal antibody (MAb), EA2-1 (213Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 μCi 213Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, 213Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.

AB - Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting 213Bi-labeled monoclonal antibody (MAb), EA2-1 (213Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 μCi 213Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, 213Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.

UR - http://www.scopus.com/inward/record.url?scp=84896877101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896877101&partnerID=8YFLogxK

U2 - 10.1128/AAC.01266-13

DO - 10.1128/AAC.01266-13

M3 - Article

VL - 58

SP - 1813

EP - 1815

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 3

ER -