Microautophagy of Cytosolic Proteins by Late Endosomes

Ranjit Sahu, Susmita Kaushik, Cristina C. Clement, Elvira S. Cannizzo, Brian Scharf, Antonia Follenzi, Ilaria Potolicchio, Edward Nieves, Ana Maria Cuervo, Laura Santambrogio

Research output: Contribution to journalArticle

333 Citations (Scopus)

Abstract

Autophagy delivers cytosolic components to lysosomes for their degradation. The delivery of autophagic cargo to late endosomes for complete or partial degradation has also been described. In this report we present evidence that distinct autophagic mechanisms control cytosolic protein delivery to late endosomes and identify a microautophagy-like process that delivers soluble cytosolic proteins to the vesicles of late endosomes/multivesicular bodies (MVBs). This microautophagy-like process has selectivity and is distinct from chaperone-mediated autophagy that occurs in lysosomes. Endosomal microautophagy occurs during MVB formation, relying on the ESCRT I and III systems for formation of the vesicles in which the cytosolic cargo is internalized. Protein cargo selection is mediated by the chaperone hsc70 and requires the cationic domain of hsc70 for electrostatic interactions with the endosomal membrane. Therefore, we propose that endosomal microautophagy shares molecular components with both the endocytic and autophagic pathways.

Original languageEnglish (US)
Pages (from-to)131-139
Number of pages9
JournalDevelopmental Cell
Volume20
Issue number1
DOIs
StatePublished - Jan 18 2011

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Endosomes
Endosomal Sorting Complexes Required for Transport
Multivesicular Bodies
Autophagy
Lysosomes
Degradation
Proteins
Coulomb interactions
Static Electricity
Membranes

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Microautophagy of Cytosolic Proteins by Late Endosomes. / Sahu, Ranjit; Kaushik, Susmita; Clement, Cristina C.; Cannizzo, Elvira S.; Scharf, Brian; Follenzi, Antonia; Potolicchio, Ilaria; Nieves, Edward; Cuervo, Ana Maria; Santambrogio, Laura.

In: Developmental Cell, Vol. 20, No. 1, 18.01.2011, p. 131-139.

Research output: Contribution to journalArticle

Sahu, R, Kaushik, S, Clement, CC, Cannizzo, ES, Scharf, B, Follenzi, A, Potolicchio, I, Nieves, E, Cuervo, AM & Santambrogio, L 2011, 'Microautophagy of Cytosolic Proteins by Late Endosomes', Developmental Cell, vol. 20, no. 1, pp. 131-139. https://doi.org/10.1016/j.devcel.2010.12.003
Sahu R, Kaushik S, Clement CC, Cannizzo ES, Scharf B, Follenzi A et al. Microautophagy of Cytosolic Proteins by Late Endosomes. Developmental Cell. 2011 Jan 18;20(1):131-139. https://doi.org/10.1016/j.devcel.2010.12.003
Sahu, Ranjit ; Kaushik, Susmita ; Clement, Cristina C. ; Cannizzo, Elvira S. ; Scharf, Brian ; Follenzi, Antonia ; Potolicchio, Ilaria ; Nieves, Edward ; Cuervo, Ana Maria ; Santambrogio, Laura. / Microautophagy of Cytosolic Proteins by Late Endosomes. In: Developmental Cell. 2011 ; Vol. 20, No. 1. pp. 131-139.
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