Mice carrying a truncated ABC gene have diminished gastric epithelial proliferation, gastric inflammation, and humoral immunity in response to Helicobacter felis infection

James G. Fox, Charles A. Dangler, Mark T. Whary, Winfried Edelmann, Raju Kucherlapati, Timothy C. Wang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Helicobacter pylori infection and adenomatous polyposis coli (Ape) gene mutations have been linked to gastric cancer in humans, but possible synergistic interaction(s) between these risk factors have not been examined. Fourteen C57BL/6 wild-type and 14 Apc1638 heterozygous mice were inoculated with Helicobacter felis at 6 weeks of age and compared at various time points with a similar number of uninfected control mice of the same genotype. Both infected and uninfected Apc1638 mice had a limited incidence of atypical proliferation fuel in the mucosa of the antrum and pyloric junction at 4.5 and 6 months of age, whereas polyps of the antrum and pylorus were present in all mice, regardless of infection status, at 7.5 months. In contrast, no altered gastric mucosal loci were observed in control or infected C57BL/6 mice at any time point. Interestingly, the infected Apc1638 mice had less epithelial proliferation and inflammation in the body of the stomach, lower anti-H. felis serum IgG antibody responses (although both the wild-type and Apc mutant mice had a Th1-like immune response, based on a predominantly IgG2a immunoglobulin response), and higher bacteria and urease scores than did infected wild-type C57BL/6 mice. In conclusion, the Apc1638 truncating mutation leads to gastric dysplasia and polyposis of the antrum and pyloric junction, but H. felis infection of the Ape mutant mouse does not lead to an increased rate of gastric neoplasia. In addition, our data suggest this Ape mutation may actually lead to decreased immune, inflammatory, and gastric hyperplastic responses to Helicobacter infection, suggesting the possibility of a novel role for this tumor suppressor gene in the immune and local tissue responses to gastric bacterial infection.

Original languageEnglish (US)
Pages (from-to)3972-3978
Number of pages7
JournalCancer Research
Volume57
Issue number18
StatePublished - Sep 15 1997

Fingerprint

Helicobacter felis
Helicobacter Infections
Humoral Immunity
Stomach
Inflammation
Hominidae
Genes
Pyloric Antrum
Inbred C57BL Mouse
Mutation
APC Genes
Urease
Pylorus
Polyps
Tumor Suppressor Genes
Bacterial Infections
Helicobacter pylori
Stomach Neoplasms
Antibody Formation
Immunoglobulins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Mice carrying a truncated ABC gene have diminished gastric epithelial proliferation, gastric inflammation, and humoral immunity in response to Helicobacter felis infection. / Fox, James G.; Dangler, Charles A.; Whary, Mark T.; Edelmann, Winfried; Kucherlapati, Raju; Wang, Timothy C.

In: Cancer Research, Vol. 57, No. 18, 15.09.1997, p. 3972-3978.

Research output: Contribution to journalArticle

Fox, James G. ; Dangler, Charles A. ; Whary, Mark T. ; Edelmann, Winfried ; Kucherlapati, Raju ; Wang, Timothy C. / Mice carrying a truncated ABC gene have diminished gastric epithelial proliferation, gastric inflammation, and humoral immunity in response to Helicobacter felis infection. In: Cancer Research. 1997 ; Vol. 57, No. 18. pp. 3972-3978.
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abstract = "Helicobacter pylori infection and adenomatous polyposis coli (Ape) gene mutations have been linked to gastric cancer in humans, but possible synergistic interaction(s) between these risk factors have not been examined. Fourteen C57BL/6 wild-type and 14 Apc1638 heterozygous mice were inoculated with Helicobacter felis at 6 weeks of age and compared at various time points with a similar number of uninfected control mice of the same genotype. Both infected and uninfected Apc1638 mice had a limited incidence of atypical proliferation fuel in the mucosa of the antrum and pyloric junction at 4.5 and 6 months of age, whereas polyps of the antrum and pylorus were present in all mice, regardless of infection status, at 7.5 months. In contrast, no altered gastric mucosal loci were observed in control or infected C57BL/6 mice at any time point. Interestingly, the infected Apc1638 mice had less epithelial proliferation and inflammation in the body of the stomach, lower anti-H. felis serum IgG antibody responses (although both the wild-type and Apc mutant mice had a Th1-like immune response, based on a predominantly IgG2a immunoglobulin response), and higher bacteria and urease scores than did infected wild-type C57BL/6 mice. In conclusion, the Apc1638 truncating mutation leads to gastric dysplasia and polyposis of the antrum and pyloric junction, but H. felis infection of the Ape mutant mouse does not lead to an increased rate of gastric neoplasia. In addition, our data suggest this Ape mutation may actually lead to decreased immune, inflammatory, and gastric hyperplastic responses to Helicobacter infection, suggesting the possibility of a novel role for this tumor suppressor gene in the immune and local tissue responses to gastric bacterial infection.",
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