MHC II expression in the cns after long-term demyelination

Barbara Cannella, Dennis A. Aquino, Cedric S. Raine

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The ability of chronically demyelinated central nervous system (CNS) tissue to express major histocompatibility complex (MHC) class II molecules has been measured in mouse spinal cord cultures exposed for 1 and 3 weeks to demye-linating anti-white matter (WM) serum. From previous studies, it was known that after 3 weeks of demyelination in vitro, such cultures are incapable of remyelination. In the present report, MHC II levels were evaluated by immunocytochemistry and by Western and Northern blots. The results have shown that after both 1 and 3 weeks of exposure to myelinotoxic anti-WM serum, the cultures retained the ability to express MHC II and this could be further upregulated by incubation with interferon 7 (IFN-y). Control groups showed increased expression of MHC II with age. By immunocytochemistry, all groups of cultures expressed high levels of MHC II and all groups showed upregulation after IFN7 treatment. Anti-WM-treated cultures demonstrated slightly higher levels of MHC II than controls. Morphologically, the MHC II expression was associated with the surface of astrocytes. Semiquantitative analysis by Western blotting confirmed the increase in class II MHC expression in the long-term treated cultures after IFN-y exposure, revealing no differences between anti-WM-treated and complement-treated cultures. This was also supported by Northern blotting which showed similar mRNA levels in both groups. These findings suggest that long-term demyelinated CNS tissue still possesses the ability to interact with CD4+ T cells, observations of significance to the expansion of the chronic multiple sclerosis lesion.

Original languageEnglish (US)
Pages (from-to)521-530
Number of pages10
JournalJournal of Neuropathology and Experimental Neurology
Volume54
Issue number4
StatePublished - 1995

Fingerprint

Demyelinating Diseases
Major Histocompatibility Complex
Nerve Tissue
Northern Blotting
Central Nervous System
Western Blotting
Immunohistochemistry
Serum
Astrocytes
Interferons
Multiple Sclerosis
Spinal Cord
Up-Regulation
T-Lymphocytes
Control Groups
Messenger RNA
White Matter

Keywords

  • Astrocytes
  • Class ii MHC
  • Demyelination
  • Multiple sclerosis
  • Western and northern blotting

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Cellular and Molecular Neuroscience
  • Neurology
  • Neuroscience(all)

Cite this

MHC II expression in the cns after long-term demyelination. / Cannella, Barbara; Aquino, Dennis A.; Raine, Cedric S.

In: Journal of Neuropathology and Experimental Neurology, Vol. 54, No. 4, 1995, p. 521-530.

Research output: Contribution to journalArticle

Cannella, B, Aquino, DA & Raine, CS 1995, 'MHC II expression in the cns after long-term demyelination', Journal of Neuropathology and Experimental Neurology, vol. 54, no. 4, pp. 521-530.
Cannella, Barbara ; Aquino, Dennis A. ; Raine, Cedric S. / MHC II expression in the cns after long-term demyelination. In: Journal of Neuropathology and Experimental Neurology. 1995 ; Vol. 54, No. 4. pp. 521-530.
@article{23ca5e76081a472983e269eea5e8bb76,
title = "MHC II expression in the cns after long-term demyelination",
abstract = "The ability of chronically demyelinated central nervous system (CNS) tissue to express major histocompatibility complex (MHC) class II molecules has been measured in mouse spinal cord cultures exposed for 1 and 3 weeks to demye-linating anti-white matter (WM) serum. From previous studies, it was known that after 3 weeks of demyelination in vitro, such cultures are incapable of remyelination. In the present report, MHC II levels were evaluated by immunocytochemistry and by Western and Northern blots. The results have shown that after both 1 and 3 weeks of exposure to myelinotoxic anti-WM serum, the cultures retained the ability to express MHC II and this could be further upregulated by incubation with interferon 7 (IFN-y). Control groups showed increased expression of MHC II with age. By immunocytochemistry, all groups of cultures expressed high levels of MHC II and all groups showed upregulation after IFN7 treatment. Anti-WM-treated cultures demonstrated slightly higher levels of MHC II than controls. Morphologically, the MHC II expression was associated with the surface of astrocytes. Semiquantitative analysis by Western blotting confirmed the increase in class II MHC expression in the long-term treated cultures after IFN-y exposure, revealing no differences between anti-WM-treated and complement-treated cultures. This was also supported by Northern blotting which showed similar mRNA levels in both groups. These findings suggest that long-term demyelinated CNS tissue still possesses the ability to interact with CD4+ T cells, observations of significance to the expansion of the chronic multiple sclerosis lesion.",
keywords = "Astrocytes, Class ii MHC, Demyelination, Multiple sclerosis, Western and northern blotting",
author = "Barbara Cannella and Aquino, {Dennis A.} and Raine, {Cedric S.}",
year = "1995",
language = "English (US)",
volume = "54",
pages = "521--530",
journal = "American Journal of Psychotherapy",
issn = "0002-9564",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - MHC II expression in the cns after long-term demyelination

AU - Cannella, Barbara

AU - Aquino, Dennis A.

AU - Raine, Cedric S.

PY - 1995

Y1 - 1995

N2 - The ability of chronically demyelinated central nervous system (CNS) tissue to express major histocompatibility complex (MHC) class II molecules has been measured in mouse spinal cord cultures exposed for 1 and 3 weeks to demye-linating anti-white matter (WM) serum. From previous studies, it was known that after 3 weeks of demyelination in vitro, such cultures are incapable of remyelination. In the present report, MHC II levels were evaluated by immunocytochemistry and by Western and Northern blots. The results have shown that after both 1 and 3 weeks of exposure to myelinotoxic anti-WM serum, the cultures retained the ability to express MHC II and this could be further upregulated by incubation with interferon 7 (IFN-y). Control groups showed increased expression of MHC II with age. By immunocytochemistry, all groups of cultures expressed high levels of MHC II and all groups showed upregulation after IFN7 treatment. Anti-WM-treated cultures demonstrated slightly higher levels of MHC II than controls. Morphologically, the MHC II expression was associated with the surface of astrocytes. Semiquantitative analysis by Western blotting confirmed the increase in class II MHC expression in the long-term treated cultures after IFN-y exposure, revealing no differences between anti-WM-treated and complement-treated cultures. This was also supported by Northern blotting which showed similar mRNA levels in both groups. These findings suggest that long-term demyelinated CNS tissue still possesses the ability to interact with CD4+ T cells, observations of significance to the expansion of the chronic multiple sclerosis lesion.

AB - The ability of chronically demyelinated central nervous system (CNS) tissue to express major histocompatibility complex (MHC) class II molecules has been measured in mouse spinal cord cultures exposed for 1 and 3 weeks to demye-linating anti-white matter (WM) serum. From previous studies, it was known that after 3 weeks of demyelination in vitro, such cultures are incapable of remyelination. In the present report, MHC II levels were evaluated by immunocytochemistry and by Western and Northern blots. The results have shown that after both 1 and 3 weeks of exposure to myelinotoxic anti-WM serum, the cultures retained the ability to express MHC II and this could be further upregulated by incubation with interferon 7 (IFN-y). Control groups showed increased expression of MHC II with age. By immunocytochemistry, all groups of cultures expressed high levels of MHC II and all groups showed upregulation after IFN7 treatment. Anti-WM-treated cultures demonstrated slightly higher levels of MHC II than controls. Morphologically, the MHC II expression was associated with the surface of astrocytes. Semiquantitative analysis by Western blotting confirmed the increase in class II MHC expression in the long-term treated cultures after IFN-y exposure, revealing no differences between anti-WM-treated and complement-treated cultures. This was also supported by Northern blotting which showed similar mRNA levels in both groups. These findings suggest that long-term demyelinated CNS tissue still possesses the ability to interact with CD4+ T cells, observations of significance to the expansion of the chronic multiple sclerosis lesion.

KW - Astrocytes

KW - Class ii MHC

KW - Demyelination

KW - Multiple sclerosis

KW - Western and northern blotting

UR - http://www.scopus.com/inward/record.url?scp=0029078172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029078172&partnerID=8YFLogxK

M3 - Article

C2 - 7602325

AN - SCOPUS:0029078172

VL - 54

SP - 521

EP - 530

JO - American Journal of Psychotherapy

JF - American Journal of Psychotherapy

SN - 0002-9564

IS - 4

ER -