MG-1

A specificity identifying members of the macrophage and granulocyte lineages of mice

Joan W. Berman, R. S. Basch

Research output: Contribution to journalArticle

Abstract

An antigen (MG-1) which behaves as a lineage marker for immature granulocytes and cells of the monocytic series in mice is described. It is identified by rabbit antimouse brain antiserum, which has been exhaustively absorbed with thymocytes, red blood cells, and a Thy-1 negative variant of a T-cell lymphoma. MG-1 is present on immature granulocytes, declines in its surface expression as the cells differentiate and is absent from the most mature cells (segmented) of the series. Early monocytes are strongly positive for MG-1 and, with maturity, the amount of cell surface antigen increases. Adherent, phagocytic macrophages are brilliantly positive when stained with anti-MG-1 antiserum in an immunofluorescence assay. The antigen is present on most of the adherent cells of the lung, spleen and peritoneum. Many multipotential stem cells also express low levels of this antigen as do some bone marrow B cells.

Original languageEnglish (US)
Pages (from-to)43-55
Number of pages13
JournalExperimental Hematology
Volume11
Issue number1
StatePublished - 1983
Externally publishedYes

Fingerprint

Granulocytes
Macrophages
Antigens
Immune Sera
T-Cell Lymphoma
Peritoneum
Thymocytes
Surface Antigens
Bone Marrow Cells
Fluorescent Antibody Technique
Monocytes
B-Lymphocytes
Stem Cells
Spleen
Erythrocytes
Rabbits
Lung
Brain

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

MG-1 : A specificity identifying members of the macrophage and granulocyte lineages of mice. / Berman, Joan W.; Basch, R. S.

In: Experimental Hematology, Vol. 11, No. 1, 1983, p. 43-55.

Research output: Contribution to journalArticle

@article{a8b42819d8204ae7a625894b6053a763,
title = "MG-1: A specificity identifying members of the macrophage and granulocyte lineages of mice",
abstract = "An antigen (MG-1) which behaves as a lineage marker for immature granulocytes and cells of the monocytic series in mice is described. It is identified by rabbit antimouse brain antiserum, which has been exhaustively absorbed with thymocytes, red blood cells, and a Thy-1 negative variant of a T-cell lymphoma. MG-1 is present on immature granulocytes, declines in its surface expression as the cells differentiate and is absent from the most mature cells (segmented) of the series. Early monocytes are strongly positive for MG-1 and, with maturity, the amount of cell surface antigen increases. Adherent, phagocytic macrophages are brilliantly positive when stained with anti-MG-1 antiserum in an immunofluorescence assay. The antigen is present on most of the adherent cells of the lung, spleen and peritoneum. Many multipotential stem cells also express low levels of this antigen as do some bone marrow B cells.",
author = "Berman, {Joan W.} and Basch, {R. S.}",
year = "1983",
language = "English (US)",
volume = "11",
pages = "43--55",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - MG-1

T2 - A specificity identifying members of the macrophage and granulocyte lineages of mice

AU - Berman, Joan W.

AU - Basch, R. S.

PY - 1983

Y1 - 1983

N2 - An antigen (MG-1) which behaves as a lineage marker for immature granulocytes and cells of the monocytic series in mice is described. It is identified by rabbit antimouse brain antiserum, which has been exhaustively absorbed with thymocytes, red blood cells, and a Thy-1 negative variant of a T-cell lymphoma. MG-1 is present on immature granulocytes, declines in its surface expression as the cells differentiate and is absent from the most mature cells (segmented) of the series. Early monocytes are strongly positive for MG-1 and, with maturity, the amount of cell surface antigen increases. Adherent, phagocytic macrophages are brilliantly positive when stained with anti-MG-1 antiserum in an immunofluorescence assay. The antigen is present on most of the adherent cells of the lung, spleen and peritoneum. Many multipotential stem cells also express low levels of this antigen as do some bone marrow B cells.

AB - An antigen (MG-1) which behaves as a lineage marker for immature granulocytes and cells of the monocytic series in mice is described. It is identified by rabbit antimouse brain antiserum, which has been exhaustively absorbed with thymocytes, red blood cells, and a Thy-1 negative variant of a T-cell lymphoma. MG-1 is present on immature granulocytes, declines in its surface expression as the cells differentiate and is absent from the most mature cells (segmented) of the series. Early monocytes are strongly positive for MG-1 and, with maturity, the amount of cell surface antigen increases. Adherent, phagocytic macrophages are brilliantly positive when stained with anti-MG-1 antiserum in an immunofluorescence assay. The antigen is present on most of the adherent cells of the lung, spleen and peritoneum. Many multipotential stem cells also express low levels of this antigen as do some bone marrow B cells.

UR - http://www.scopus.com/inward/record.url?scp=0020696015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020696015&partnerID=8YFLogxK

M3 - Article

VL - 11

SP - 43

EP - 55

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 1

ER -