Methylxanthine inhibit fungal chitinases and exhibit antifungal activity.

Kalliope Tsirilakis, Christy Kim, Alfin G. Vicencio, Christopher Andrade, Arturo Casadevall, David L. Goldman

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Chitinases are necessary for fungal cell wall remodeling and cell replication. Methylxanthines have been shown to competitively inhibit family 18 chitinases in vitro. We sought to determine the effects of methylxanthines on fungal chitinases. Fungi demonstrated variable chitinase activity and incubation with methylxanthines (0.5-10 mM) resulted in a dose-dependent decrease in this activity. All fungi tested, except for Candida spp., demonstrated growth inhibition in the presence of methylxanthines at a concentration of 10 mM. India ink staining demonstrated impaired budding and decreased cell size for methylxanthine-treated Cryptococcus neoformans. C. neoformans and Aspergillus fumigatus treated with pentoxifylline also exhibited abnormal cell morphology. In addition, pentoxifylline-treated C. neoformans exhibited increased susceptibility to calcofluor and a leaky melanin phenotype consistent with defective cell wall function. Our data suggest that a variety of fungi express chitinases and that methylxanthines have antifungal properties related to their inhibition of fungal chitinases. Our results highlight the potential utility of targeting chitinases in the development of novel antifungal therapies.

Original languageEnglish (US)
Pages (from-to)83-91
Number of pages9
JournalMycopathologia
Volume173
Issue number2-3
DOIs
StatePublished - Mar 2012
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science
  • veterinary (miscalleneous)

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    Tsirilakis, K., Kim, C., Vicencio, A. G., Andrade, C., Casadevall, A., & Goldman, D. L. (2012). Methylxanthine inhibit fungal chitinases and exhibit antifungal activity. Mycopathologia, 173(2-3), 83-91. https://doi.org/10.1007/s11046-011-9483-x