Methods to study interactions between ciliogenesis and autophagy

Birgit H. Satir, Olatz Pampliega

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Autophagy is a catabolic pathway for the degradation and recycling of intracellular components, contributing to maintain cell homeostasis. Changes in autophagy activity can be monitored by a variety of biochemical and functional assays that should be used in combination. Recently, it has been described that signaling from the primary cilium modulates autophagy. This novel and reciprocal interaction will impact diverse aspects of the cell biology in healthy and pathophysiological conditions. Here, we describe methods to monitor autophagy activity in cilia mutants, as well as the use of autophagy mutants to monitor ciliogenesis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages53-67
Number of pages15
Volume1454
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1454
ISSN (Print)10643745

Keywords

  • Autophagy
  • Immunofluorescence
  • LC3-II
  • MCherry-GFP-LC3
  • Primary cilia
  • Western blotting

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Methods to study interactions between ciliogenesis and autophagy'. Together they form a unique fingerprint.

  • Cite this

    Satir, B. H., & Pampliega, O. (2016). Methods to study interactions between ciliogenesis and autophagy. In Methods in Molecular Biology (Vol. 1454, pp. 53-67). (Methods in Molecular Biology; Vol. 1454). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3789-9_4