TY - CHAP
T1 - Methods to Study Chaperone-Mediated Autophagy
AU - Arias, E.
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017
Y1 - 2017
N2 - Chaperone-mediated autophagy (CMA), a selective form of degradation of cytosolic proteins in lysosomes, contributes to maintenance of proteostasis and to the cellular adaptation to stress. CMA substrates are selectively recognized and delivered by a cytosolic chaperone to the lysosomal surface, where, upon unfolding, they are internalized through a membrane translocation complex. Defective or dysfunctional CMA has been associated with human pathologies such as neurodegeneration, cancer, immunodeficiency, or diabetes, increasing the overall interest in methods to monitor this selective autophagic pathway. In this chapter, we review the different experimental approaches used to evaluate CMA activity in different organs from animals or in cell cultures in vitro.
AB - Chaperone-mediated autophagy (CMA), a selective form of degradation of cytosolic proteins in lysosomes, contributes to maintenance of proteostasis and to the cellular adaptation to stress. CMA substrates are selectively recognized and delivered by a cytosolic chaperone to the lysosomal surface, where, upon unfolding, they are internalized through a membrane translocation complex. Defective or dysfunctional CMA has been associated with human pathologies such as neurodegeneration, cancer, immunodeficiency, or diabetes, increasing the overall interest in methods to monitor this selective autophagic pathway. In this chapter, we review the different experimental approaches used to evaluate CMA activity in different organs from animals or in cell cultures in vitro.
KW - Autophagy
KW - Chemical modulators
KW - Fluorescent reporters
KW - Lysosomes
KW - Organelle isolation
UR - http://www.scopus.com/inward/record.url?scp=85006961534&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85006961534&partnerID=8YFLogxK
U2 - 10.1016/bs.mie.2016.10.009
DO - 10.1016/bs.mie.2016.10.009
M3 - Chapter
C2 - 28237106
AN - SCOPUS:85006961534
T3 - Methods in Enzymology
SP - 283
EP - 305
BT - Methods in Enzymology
PB - Academic Press Inc.
ER -