Methods to study chaperone-mediated autophagy

Bindi Patel, Ana Maria Cuervo

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Cytosolic substrates are selectively identified and targeted by chaperones to lysosomes where they are subsequently translocated into the organelle lumen through a dedicated CMA-associated lysosomal membrane receptor/translocation complex. CMA contributes to maintaining a functional proteome, through elimination of altered proteins, and participates in the cellular energetic balance through amino acid recycling. Defective or dysfunctional CMA has been associated with human pathologies such as neurodegeneration, cancer, immunodeficiency or diabetes, increasing the overall interest in methods to monitor this selective autophagic pathway. Here, we describe approaches used to study CMA in different experimental models.

Original languageEnglish (US)
Pages (from-to)133-140
Number of pages8
JournalMethods
Volume75
DOIs
StatePublished - Mar 15 2015

Keywords

  • Autophagy
  • Fluorescent reporters
  • Lysosomes
  • Organelle isolation
  • Proteolysis

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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