Methods for the enzymatic synthesis of tyrosine and phenylalanine labeled with nitrogen-13

Alan S. Gelbard, Arthur J L Cooper, Yasuhisa Asano, Edward Nieves, Sabina Filc-Dericco, Karen C. Rosenspire

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

l-[13N]Tyrosine and l-[13N]phenylalanine were synthesized using immobilized enzymes by two methods. In method 1, [13N]ammonia is converted to l-[13N]glutamate; transamination with p-hydroxyphenylpyruvate yields l-[13N]tyrosine. [13N]Tyrosine is separated from other labeled intermediates on a Poropak Q column. In method 2, phenylalanine dehydrogenase catalyzes the reversible reductive [13N]amination of either phenylpyruvate or p-hydroxyphenylpyruvate to form l-[13N]phenylalanine or l-[13N]tyrosine, respectively. The feasibility of labeling DOPA and tryptophan with 13N was also demonstrated.

Original languageEnglish (US)
Pages (from-to)229-233
Number of pages5
JournalInternational Journal of Radiation Applications and Instrumentation. Part
Volume41
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

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phenylalanine
tyrosine
nitrogen
synthesis
glutamates
dehydrogenases
tryptophan
marking
enzymes
ammonia

ASJC Scopus subject areas

  • Radiation

Cite this

Methods for the enzymatic synthesis of tyrosine and phenylalanine labeled with nitrogen-13. / Gelbard, Alan S.; Cooper, Arthur J L; Asano, Yasuhisa; Nieves, Edward; Filc-Dericco, Sabina; Rosenspire, Karen C.

In: International Journal of Radiation Applications and Instrumentation. Part, Vol. 41, No. 2, 1990, p. 229-233.

Research output: Contribution to journalArticle

Gelbard, Alan S. ; Cooper, Arthur J L ; Asano, Yasuhisa ; Nieves, Edward ; Filc-Dericco, Sabina ; Rosenspire, Karen C. / Methods for the enzymatic synthesis of tyrosine and phenylalanine labeled with nitrogen-13. In: International Journal of Radiation Applications and Instrumentation. Part. 1990 ; Vol. 41, No. 2. pp. 229-233.
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AU - Filc-Dericco, Sabina

AU - Rosenspire, Karen C.

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