Methodologies to decipher the cell secretome

Paromita Mukherjee, Sridhar Mani

Research output: Contribution to journalReview articlepeer-review

56 Scopus citations


The cell secretome is a collection of proteins consisting of transmembrane proteins (TM) and proteins secreted by cells into the extracellular space. A significant portion (~ 13-20%) of the human proteome consists of secretory proteins. The secretory proteins play important roles in cell migration, cell signaling and communication. There is a plethora of methodologies available like Serial Analysis of Gene Expression (SAGE), DNA microarrays, antibody arrays and bead-based arrays, mass spectrometry, RNA sequencing and yeast, bacterial and mammalian secretion traps to identify the cell secretomes. There are many advantages and disadvantages in using any of the above methods. This review aims to discuss the methodologies available along with their potential advantages and disadvantages to identify secretory proteins. This review is a part of a Special issue on The Secretome. This article is part of a Special Issue entitled: An Updated Secretome.

Original languageEnglish (US)
Pages (from-to)2226-2232
Number of pages7
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Issue number11
StatePublished - 2013


  • Secretome
  • Secretory protein
  • Signal peptides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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