Metazoan Nuclear Pores Provide a Scaffold for Poised Genes and Mediate Induced Enhancer-Promoter Contacts

Pau Pascual-Garcia, Brian Debo, Jennifer R. Aleman, Jessica A. Talamas, Yemin Lan, Nha H. Nguyen, Kyoung J. Won, Maya Capelson

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Nuclear pore complex components (Nups) have been implicated in transcriptional regulation, yet what regulatory steps are controlled by metazoan Nups remains unclear. We identified the presence of multiple Nups at promoters, enhancers, and insulators in the Drosophila genome. In line with this binding, we uncovered a functional role for Nup98 in mediating enhancer-promoter looping at ecdysone-inducible genes. These genes were found to be stably associated with nuclear pores before and after activation. Although changing levels of Nup98 disrupted enhancer-promoter contacts, it did not affect ongoing transcription but instead compromised subsequent transcriptional activation or transcriptional memory. In support of the enhancer-looping role, we found Nup98 to gain and retain physical interactions with architectural proteins upon stimulation with ecdysone. Together, our data identify Nups as a class of architectural proteins for enhancers and supports a model in which animal genomes use the nuclear pore as an organizing scaffold for inducible poised genes.

Original languageEnglish (US)
Pages (from-to)63-76.e6
JournalMolecular Cell
Volume66
Issue number1
DOIs
StatePublished - Apr 6 2017

Keywords

  • Nup98
  • enhancer
  • genome architecture
  • looping
  • nuclear organization
  • nuclear pore complex
  • nucleoporin
  • poised genes
  • transcription
  • transcriptional memory

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Metazoan Nuclear Pores Provide a Scaffold for Poised Genes and Mediate Induced Enhancer-Promoter Contacts'. Together they form a unique fingerprint.

  • Cite this

    Pascual-Garcia, P., Debo, B., Aleman, J. R., Talamas, J. A., Lan, Y., Nguyen, N. H., Won, K. J., & Capelson, M. (2017). Metazoan Nuclear Pores Provide a Scaffold for Poised Genes and Mediate Induced Enhancer-Promoter Contacts. Molecular Cell, 66(1), 63-76.e6. https://doi.org/10.1016/j.molcel.2017.02.020