TY - JOUR
T1 - Metaphase FISHing of transgenic mice recommended
T2 - FISH and SKY define BAC-mediated balanced translocation
AU - Abrahams, Brett S.
AU - Chong, Annabelle C.O.
AU - Nisha, Maimun
AU - Milette, Danielle
AU - Brewster, David A.
AU - Berry, Melissa L.
AU - Muratkhodjaev, Farkhad
AU - Mai, Sabine
AU - Rajcan-Separovic, Evica
AU - Simpson, Elizabeth M.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - The evolving trend to use larger transgenes and their associated increased chance of unexpected genetic events mandates more careful characterization of transgenic mice. In characterizing our five new mouse strains transgenic for the BAC, bEMS4, we have identified the highest copy number reported to date: the stable incorporation of -40 copies of a 194-kb expressed transgene in a single insertion site. We caution, however, that standard molecular techniques failed to identify a balanced translocation in another strain, and an inappropriate site of insertion in a third. Molecular cytogenetic analysis using metaphase FISH was the minimum level of characterization needed to reveal these unexpected genetic events. In addition, we combined FISH and SKY to identify the transgene at the breakpoints of the balanced translocation, t(3;9). This is the first description of a BAC-mediated chromosomal rearrangement and the first application of SKY to identify transgene-induced chromosomal rearrangements.
AB - The evolving trend to use larger transgenes and their associated increased chance of unexpected genetic events mandates more careful characterization of transgenic mice. In characterizing our five new mouse strains transgenic for the BAC, bEMS4, we have identified the highest copy number reported to date: the stable incorporation of -40 copies of a 194-kb expressed transgene in a single insertion site. We caution, however, that standard molecular techniques failed to identify a balanced translocation in another strain, and an inappropriate site of insertion in a third. Molecular cytogenetic analysis using metaphase FISH was the minimum level of characterization needed to reveal these unexpected genetic events. In addition, we combined FISH and SKY to identify the transgene at the breakpoints of the balanced translocation, t(3;9). This is the first description of a BAC-mediated chromosomal rearrangement and the first application of SKY to identify transgene-induced chromosomal rearrangements.
KW - Bacterial artificial chromosome
KW - Balanced translocation
KW - Breakpoints
KW - FISH
KW - Nr2e1
KW - Orphan receptor
KW - SKY
KW - Tailless
KW - Transgenic mice
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U2 - 10.1002/gene.10205
DO - 10.1002/gene.10205
M3 - Article
C2 - 12872244
AN - SCOPUS:10744225588
SN - 1526-954X
VL - 36
SP - 134
EP - 141
JO - Genesis (United States)
JF - Genesis (United States)
IS - 3
ER -