Metal thiolate coordination in the E7 proteins of human papilloma virus 16 and cottontail rabbit papilloma virus as expressed in Escherichia coli

E. Jill Roth, Boris Kurz, Lingming Liang, Craig L. Hansen, Charles T. Dameron, Dennis R. Winge, David Smotkin

Research output: Contribution to journalArticle

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Abstract

The oncogenic E7 proteins of human papilloma virus (HPV 16) and of cottontail rabbit papilloma virus (CRPV) have been purified from an expression system in Escherichia coli. The proteins as purified from E. coli contain one tightly bound Zn(II) ion per molecule. The metal site shows facile exchange with either Cd(II) or Cu(I). The HPV 16 E7 maximally bound one Cd(II) or two Cu(I) ions, while the CRPV E7 bound two Cd(II) or three Cu(I) ions. The Cd(II) and Cu(I) E7 molecules exhibited optical transitions in the ultraviolet suggestive of metal:thiolate coordination. E7 proteins from HPV 16 and CRPV contain 7 and 8 cysteines/molecule, respectively. Reaction of the E7 proteins with the sulfhydryl reagent, dithiodipyridine, revealed that all the cysteinyl sulfurs are present in the reduced thiol state. Cu(I)-E7 molecules are luminescent with maximal emission at 570 nm. The observed emission at room temperature is indicative of metal coordination within a compact protein environment shielded from solvent interactions. The emission maxima occurs at the same wavelength (570 nm) as Cu(I)-cysteinyl sulfur clusters in Cu(I)-metallothioneins. The single Zn(II) atom in each protein can be removed from E7 in the presence of EDTA. The resulting apoE7 molecules remain soluble and can be partially reconstituted with Cd(II) to regain the ultraviolet charge transfer transitions.

Original languageEnglish (US)
Pages (from-to)16390-16395
Number of pages6
JournalJournal of Biological Chemistry
Volume267
Issue number23
StatePublished - Aug 15 1992
Externally publishedYes

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Papillomaviridae
Papilloma
Viruses
Escherichia coli
Metals
Rabbits
Molecules
Human papillomavirus 16
Ions
Proteins
Sulfur
Sulfhydryl Reagents
Regain
Optical transitions
Metallothionein
Sulfhydryl Compounds
Edetic Acid
Cysteine
Charge transfer
Wavelength

ASJC Scopus subject areas

  • Biochemistry

Cite this

Metal thiolate coordination in the E7 proteins of human papilloma virus 16 and cottontail rabbit papilloma virus as expressed in Escherichia coli. / Jill Roth, E.; Kurz, Boris; Liang, Lingming; Hansen, Craig L.; Dameron, Charles T.; Winge, Dennis R.; Smotkin, David.

In: Journal of Biological Chemistry, Vol. 267, No. 23, 15.08.1992, p. 16390-16395.

Research output: Contribution to journalArticle

Jill Roth, E. ; Kurz, Boris ; Liang, Lingming ; Hansen, Craig L. ; Dameron, Charles T. ; Winge, Dennis R. ; Smotkin, David. / Metal thiolate coordination in the E7 proteins of human papilloma virus 16 and cottontail rabbit papilloma virus as expressed in Escherichia coli. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 23. pp. 16390-16395.
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