Metabonomics approach to understanding acute and chronic stress in rat models

Xiaoyan Wang, Tie Zhao, Yunping Qiu, Mingming Su, Tao Jiang, Mingmei Zhou, Aihua Zhao, Wei Jia

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The effects of acute and chronic stress on the production of systemic metabolites were investigated in male Sprague-Dawley (SD) rats. Metabolites excreted in urine were analyzed using GC/MS in conjunction with multivariate and univariate statistical techniques. SD rats were subjected to two kinds of acute stress and chronic unpredictable mild stress, respectively. Metabolic analysis demonstrated that urinary expression of a number of metabolites including glutamate, glutamine, homovanillate, proline, succinate, citrate, and tyrosine altered in the acute stress model in the same way as in the chronic model, while pimelate and hippurate changed in the opposite trend. The results suggested that the stress induced metabolic perturbations were reversible and nonspecific. Metabolic response to chronic combined stress revealed biochemical clues to depression-like symptoms validated by behavior and physiologic results. This study provides a noninvasive and dynamic analytical strategy for the characterization of endogenous metabolic perturbations induced by external stress.

Original languageEnglish (US)
Pages (from-to)2511-2518
Number of pages8
JournalJournal of Proteome Research
Volume8
Issue number5
DOIs
StatePublished - May 1 2009
Externally publishedYes

Fingerprint

Metabolomics
Sprague Dawley Rats
Rats
Metabolites
Succinic Acid
Glutamine
Proline
Citric Acid
Tyrosine
Glutamic Acid
Urine
Depression
hippuric acid

Keywords

  • Acute stress
  • Chronic stress
  • Gas chromatography/mass spectrometry
  • Metabonomics
  • Multivariate analysis
  • Urine

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Metabonomics approach to understanding acute and chronic stress in rat models. / Wang, Xiaoyan; Zhao, Tie; Qiu, Yunping; Su, Mingming; Jiang, Tao; Zhou, Mingmei; Zhao, Aihua; Jia, Wei.

In: Journal of Proteome Research, Vol. 8, No. 5, 01.05.2009, p. 2511-2518.

Research output: Contribution to journalArticle

Wang, X, Zhao, T, Qiu, Y, Su, M, Jiang, T, Zhou, M, Zhao, A & Jia, W 2009, 'Metabonomics approach to understanding acute and chronic stress in rat models', Journal of Proteome Research, vol. 8, no. 5, pp. 2511-2518. https://doi.org/10.1021/pr801086k
Wang, Xiaoyan ; Zhao, Tie ; Qiu, Yunping ; Su, Mingming ; Jiang, Tao ; Zhou, Mingmei ; Zhao, Aihua ; Jia, Wei. / Metabonomics approach to understanding acute and chronic stress in rat models. In: Journal of Proteome Research. 2009 ; Vol. 8, No. 5. pp. 2511-2518.
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