Abstract
The structure and metabolism of the rhesus macaque brain, an advanced model for neurologic diseases and their treatment response, is often studied noninvasively with MRI and 1H-MR spectroscopy. Due to the shorter transverse relaxation time (T2) at the higher magnetic fields these studies favor, the echo times used in 1H-MR spectroscopy subject the metabolites to unknown T2 weighting, decreasing the accuracy of quantification which is key for inter- and intra-animal comparisons. To establish the "baseline" (healthy animal) T2 values, we mapped them for the three main metabolites' T2s at 3 T in four healthy rhesus macaques and tested the hypotheses that their mean values are similar (i) among animals; and (ii) to analogs regions in the human brain. This was done with three-dimensional multivoxel 1H-MR spectroscopy at (0.6 x 0.6 x 0.5 cm)3 = 180 μL spatial resolution over a 4.2 x 3.0 x 2.0 = 25 cm3 (∼30%) of the macaque brain in a two-point protocol that optimizes T2 precision per unit time. The estimated T 2s in several gray and white matter regions are all within 10% of those reported in the human brain (mean ± standard error of the mean): N-acetylaspartate = 316 ± 7, creatine = 177 ± 3, and choline = 264 ± 9 ms, with no statistically significant gray versus white matter differences.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1292-1299 |
| Number of pages | 8 |
| Journal | Magnetic Resonance in Medicine |
| Volume | 62 |
| Issue number | 5 |
| DOIs | |
| State | Published - Nov 2009 |
| Externally published | Yes |
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Keywords
- Animal models
- Brain metabolites
- High magnetic field
- MR spectroscopic imaging
- Rhesus macaque
- Transverse relaxation time
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
Cite this
Metabolite proton T2 mapping in the healthy rhesus macaque brain at 3 T. / Liu, Songtao; Gonen, Oded; Fleysher, Roman; Fleysher, Lazar; Babb, James S.; Soher, Brian J.; Joo, Chan Gyu; Ratai, Eva Maria; González, R. Gilberto.
In: Magnetic Resonance in Medicine, Vol. 62, No. 5, 11.2009, p. 1292-1299.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metabolite proton T2 mapping in the healthy rhesus macaque brain at 3 T
AU - Liu, Songtao
AU - Gonen, Oded
AU - Fleysher, Roman
AU - Fleysher, Lazar
AU - Babb, James S.
AU - Soher, Brian J.
AU - Joo, Chan Gyu
AU - Ratai, Eva Maria
AU - González, R. Gilberto
PY - 2009/11
Y1 - 2009/11
N2 - The structure and metabolism of the rhesus macaque brain, an advanced model for neurologic diseases and their treatment response, is often studied noninvasively with MRI and 1H-MR spectroscopy. Due to the shorter transverse relaxation time (T2) at the higher magnetic fields these studies favor, the echo times used in 1H-MR spectroscopy subject the metabolites to unknown T2 weighting, decreasing the accuracy of quantification which is key for inter- and intra-animal comparisons. To establish the "baseline" (healthy animal) T2 values, we mapped them for the three main metabolites' T2s at 3 T in four healthy rhesus macaques and tested the hypotheses that their mean values are similar (i) among animals; and (ii) to analogs regions in the human brain. This was done with three-dimensional multivoxel 1H-MR spectroscopy at (0.6 x 0.6 x 0.5 cm)3 = 180 μL spatial resolution over a 4.2 x 3.0 x 2.0 = 25 cm3 (∼30%) of the macaque brain in a two-point protocol that optimizes T2 precision per unit time. The estimated T 2s in several gray and white matter regions are all within 10% of those reported in the human brain (mean ± standard error of the mean): N-acetylaspartate = 316 ± 7, creatine = 177 ± 3, and choline = 264 ± 9 ms, with no statistically significant gray versus white matter differences.
AB - The structure and metabolism of the rhesus macaque brain, an advanced model for neurologic diseases and their treatment response, is often studied noninvasively with MRI and 1H-MR spectroscopy. Due to the shorter transverse relaxation time (T2) at the higher magnetic fields these studies favor, the echo times used in 1H-MR spectroscopy subject the metabolites to unknown T2 weighting, decreasing the accuracy of quantification which is key for inter- and intra-animal comparisons. To establish the "baseline" (healthy animal) T2 values, we mapped them for the three main metabolites' T2s at 3 T in four healthy rhesus macaques and tested the hypotheses that their mean values are similar (i) among animals; and (ii) to analogs regions in the human brain. This was done with three-dimensional multivoxel 1H-MR spectroscopy at (0.6 x 0.6 x 0.5 cm)3 = 180 μL spatial resolution over a 4.2 x 3.0 x 2.0 = 25 cm3 (∼30%) of the macaque brain in a two-point protocol that optimizes T2 precision per unit time. The estimated T 2s in several gray and white matter regions are all within 10% of those reported in the human brain (mean ± standard error of the mean): N-acetylaspartate = 316 ± 7, creatine = 177 ± 3, and choline = 264 ± 9 ms, with no statistically significant gray versus white matter differences.
KW - Animal models
KW - Brain metabolites
KW - High magnetic field
KW - MR spectroscopic imaging
KW - Rhesus macaque
KW - Transverse relaxation time
UR - http://www.scopus.com/inward/record.url?scp=70449427476&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70449427476&partnerID=8YFLogxK
U2 - 10.1002/mrm.22117
DO - 10.1002/mrm.22117
M3 - Article
C2 - 19780178
AN - SCOPUS:70449427476
VL - 62
SP - 1292
EP - 1299
JO - Magnetic Resonance in Medicine
JF - Magnetic Resonance in Medicine
SN - 0740-3194
IS - 5
ER -