Metabolic labeling of woodchuck hepatitis B virus X protein in naturally infected hepatocytes reveals a bimodal half-life and association with the nuclear framework

Maura Dandri, Joerg Petersen, Richard J. Stockert, Thomas M. Harris, Charles E. Rogler

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In order to identify potential sites of hepadnavirus X protein action, we have investigated the subcellular distribution and the stability of woodchuck hepatitis virus (WHV) X protein (WHx) in primary hepatocytes isolated from woodchucks with persistent WHV infection. In vivo cell labeling and cell fractionation studies showed that the majority of WHx is a soluble cytoplasmic protein while a minor part of newly synthesized WHx is associated with a nuclear framework fraction (20%) and with cytoskeletal components (5 to 10%). Pulse-chase experiments revealed that cytoplasmic WHx has a short half-life and decays with bimodal kinetics (approximately 20 min and 3 h). The rates of association and turnover of nucleus-associated WHx suggest that compartmentalization may be responsible for the bimodal turnover observed in the cytoplasm.

Original languageEnglish (US)
Pages (from-to)9359-9364
Number of pages6
JournalJournal of Virology
Volume72
Issue number11
StatePublished - Nov 1998

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Woodchuck hepatitis virus
hepatocytes
half life
Half-Life
Hepatocytes
Proteins
proteins
Hepadnaviridae
Woodchuck Hepatitis B Virus
Marmota
Cell Fractionation
Marmota monax
cell fractionation
Virus Diseases
Woodchuck hepatitis B virus protein X
Cytoplasm
cytoplasm
deterioration
kinetics

ASJC Scopus subject areas

  • Immunology

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Metabolic labeling of woodchuck hepatitis B virus X protein in naturally infected hepatocytes reveals a bimodal half-life and association with the nuclear framework. / Dandri, Maura; Petersen, Joerg; Stockert, Richard J.; Harris, Thomas M.; Rogler, Charles E.

In: Journal of Virology, Vol. 72, No. 11, 11.1998, p. 9359-9364.

Research output: Contribution to journalArticle

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