Metabolic changes in mesenchymal stem cells in osteogenic medium measured by autofluorescence spectroscopy

Johann M G Reyes, Sara Fermanian, Fan Yang, Shi You Zhou, Samantha Herretes, Douglas B. Murphy, Jennifer H. Elisseeff, Roy S. Chuck

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The purpose of this study was to measure metabolic changes in mesenchymal stem cells (MSCs) placed in osteogenic medium by autofluorescence spectroscopy. MSCs were plated in stem cell-supporting or osteogenic medium and imaged. Shift from the basic growth environment to the inductive osteogenic environment was confirmed by reverse transcription-polymerase chain reaction. Reduced pyridine nucleotides were detected by exciting near 366 nm and measuring fluorescence at 450 nm, and oxidized flavoproteins were detected by exciting at 460 nm and measuring fluorescence at 540 nm. The ratio of these fluorescence measurements, reduction-oxidation (redox) fluorometry, is a noninvasive measure of the cellular metabolic state. The detected pyridine nucleotide to flavoprotein ratio decreased upon transitioning from the stem cell to the differentiated state, as well as with increasing cell density and cell-cell contact. MSC metabolism increased upon placement in differentiating medium and with increasing cell density and contact. Redox fluorometry is a feasible, noninvasive technique for distinguishing MSCs from further differentiated cells.

Original languageEnglish (US)
Pages (from-to)1213-1217
Number of pages5
JournalStem Cells
Volume24
Issue number5
DOIs
StatePublished - May 2006
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Spectrum Analysis
Flavoproteins
Fluorometry
Fluorescence
Stem Cells
Nucleotides
Cell Count
Reverse Transcription
Oxidation-Reduction
Polymerase Chain Reaction
Growth
pyridine

Keywords

  • Bone marrow
  • Differentiation
  • Fluorescence microscopy
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Metabolic changes in mesenchymal stem cells in osteogenic medium measured by autofluorescence spectroscopy. / Reyes, Johann M G; Fermanian, Sara; Yang, Fan; Zhou, Shi You; Herretes, Samantha; Murphy, Douglas B.; Elisseeff, Jennifer H.; Chuck, Roy S.

In: Stem Cells, Vol. 24, No. 5, 05.2006, p. 1213-1217.

Research output: Contribution to journalArticle

Reyes, JMG, Fermanian, S, Yang, F, Zhou, SY, Herretes, S, Murphy, DB, Elisseeff, JH & Chuck, RS 2006, 'Metabolic changes in mesenchymal stem cells in osteogenic medium measured by autofluorescence spectroscopy', Stem Cells, vol. 24, no. 5, pp. 1213-1217. https://doi.org/10.1634/stemcells.2004-0324
Reyes, Johann M G ; Fermanian, Sara ; Yang, Fan ; Zhou, Shi You ; Herretes, Samantha ; Murphy, Douglas B. ; Elisseeff, Jennifer H. ; Chuck, Roy S. / Metabolic changes in mesenchymal stem cells in osteogenic medium measured by autofluorescence spectroscopy. In: Stem Cells. 2006 ; Vol. 24, No. 5. pp. 1213-1217.
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