Metabolic alterations to the mucosal microbiota in inflammatory bowel disease

Michael Davenport, Jordan Poles, Jacqueline M. Leung, Martin J. Wolff, Wasif M. Abidi, Thomas A. Ullman, Lloyd Mayer, Ilseung Cho, P'ng Loke

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: Inflammation during inflammatory bowel disease may alter nutrient availability to adherent mucosal bacteria and impact their metabolic function. Microbial metabolites may regulate intestinal CD4+ T-cell homeostasis. We investigated the relationship between inflammation and microbial function by inferred metagenomics of the mucosal microbiota from colonic pinch biopsies of patients with inflammatory bowel disease. Methods: Paired pinch biopsy samples of known inflammation states were analyzed from ulcerative colitis (UC) (23), Crohn's disease (CD) (21), and control (24) subjects by 16S ribosomal sequencing, histopathologic assessment, and flow cytometry. PICRUSt was used to generate metagenomic data and derive relative Kyoto Encyclopedia of Genes and Genomes Pathway abundance information. Leukocytes were isolated from paired biopsy samples and analyzed by multicolor flow cytometry. Active inflammation was defined by neutrophil infiltration into the epithelium. Results: Carriage of metabolic pathways in the mucosal microbiota was relatively stable among patients with inflammatory bowel disease, despite large variations in individual bacterial community structures. However, microbial function was significantly altered in inflamed tissue of UC patients, with a reduction in carbohydrate and nucleotide metabolism in favor of increased lipid and amino acid metabolism. These differences were not observed in samples from CD patients. In CD, microbial lipid, carbohydrate, and amino acid metabolism tightly correlated with the frequency of CD4+Foxp3+ Tregs, whereas in UC, these pathways correlated with the frequency of CD4 +IL-22+ (TH22) cells. Conclusions: Metabolic pathways of the mucosal microbiota in CD do not vary as much as UC with inflammation state, indicating a more systemic perturbation of host-bacteria interactions in CD compared with more localized dysfunction in UC.

Original languageEnglish (US)
Pages (from-to)723-731
Number of pages9
JournalInflammatory Bowel Diseases
Volume20
Issue number4
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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Microbiota
Ulcerative Colitis
Inflammatory Bowel Diseases
Crohn Disease
Inflammation
Metagenomics
Metabolic Networks and Pathways
Biopsy
Flow Cytometry
Encyclopedias
Bacteria
Lipids
Bacterial Structures
Amino Acids
Neutrophil Infiltration
Carbohydrate Metabolism
Leukocytes
Homeostasis
Epithelium
Nucleotides

ASJC Scopus subject areas

  • Immunology and Allergy
  • Gastroenterology

Cite this

Davenport, M., Poles, J., Leung, J. M., Wolff, M. J., Abidi, W. M., Ullman, T. A., ... Loke, P. (2014). Metabolic alterations to the mucosal microbiota in inflammatory bowel disease. Inflammatory Bowel Diseases, 20(4), 723-731. https://doi.org/10.1097/MIB.0000000000000011

Metabolic alterations to the mucosal microbiota in inflammatory bowel disease. / Davenport, Michael; Poles, Jordan; Leung, Jacqueline M.; Wolff, Martin J.; Abidi, Wasif M.; Ullman, Thomas A.; Mayer, Lloyd; Cho, Ilseung; Loke, P'ng.

In: Inflammatory Bowel Diseases, Vol. 20, No. 4, 01.01.2014, p. 723-731.

Research output: Contribution to journalArticle

Davenport, M, Poles, J, Leung, JM, Wolff, MJ, Abidi, WM, Ullman, TA, Mayer, L, Cho, I & Loke, P 2014, 'Metabolic alterations to the mucosal microbiota in inflammatory bowel disease', Inflammatory Bowel Diseases, vol. 20, no. 4, pp. 723-731. https://doi.org/10.1097/MIB.0000000000000011
Davenport, Michael ; Poles, Jordan ; Leung, Jacqueline M. ; Wolff, Martin J. ; Abidi, Wasif M. ; Ullman, Thomas A. ; Mayer, Lloyd ; Cho, Ilseung ; Loke, P'ng. / Metabolic alterations to the mucosal microbiota in inflammatory bowel disease. In: Inflammatory Bowel Diseases. 2014 ; Vol. 20, No. 4. pp. 723-731.
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