Mesodermal Tbx1 is required for patterning the proximal mandible in mice

Vimla S. Aggarwal, Courtney Carpenter, Laina Freyer, Jun Liao, Marilena Petti, Bernice E. Morrow

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Defects in the lower jaw, or mandible, occur commonly either as isolated malformations or in association with genetic syndromes. Understanding its formation and genetic pathways required for shaping its structure in mammalian model organisms will shed light into the pathogenesis of malformations in humans. The lower jaw is derived from the mandibular process of the first pharyngeal arch (MdPA1) during embryogenesis. Integral to the development of the mandible is the signaling interplay between Fgf8 and Bmp4 in the rostral ectoderm and their downstream effector genes in the underlying neural crest derived mesenchyme. The non-neural crest MdPA1 core mesoderm is needed to form muscles of mastication, but its role in patterning the mandible is unknown. Here, we show that mesoderm specific deletion of Tbx1, a T-box transcription factor and gene for velo-cardio-facial/DiGeorge syndrome, results in defects in formation of the proximal mandible by shifting expression of Fgf8, Bmp4 and their downstream effector genes in mouse embryos at E10.5. This occurs without significant changes in cell proliferation or apoptosis at the same stage. Our results elucidate a new function for the non-neural crest core mesoderm and specifically, mesodermal Tbx1, in shaping the lower jaw.

Original languageEnglish (US)
Pages (from-to)669-681
Number of pages13
JournalDevelopmental Biology
Volume344
Issue number2
DOIs
StatePublished - Aug 2010

Fingerprint

Mesoderm
Mandible
Jaw
DiGeorge Syndrome
Genes
Branchial Region
Ectoderm
Neural Crest
Mastication
Embryonic Development
Transcription Factors
Embryonic Structures
Cell Proliferation
Apoptosis
Muscles

Keywords

  • Mandible
  • Mandibular arch
  • Mouse model
  • Organogenesis
  • Pharyngeal apparatus
  • Tbx1
  • Velo-cardio-facial syndrome

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Mesodermal Tbx1 is required for patterning the proximal mandible in mice. / Aggarwal, Vimla S.; Carpenter, Courtney; Freyer, Laina; Liao, Jun; Petti, Marilena; Morrow, Bernice E.

In: Developmental Biology, Vol. 344, No. 2, 08.2010, p. 669-681.

Research output: Contribution to journalArticle

Aggarwal, Vimla S. ; Carpenter, Courtney ; Freyer, Laina ; Liao, Jun ; Petti, Marilena ; Morrow, Bernice E. / Mesodermal Tbx1 is required for patterning the proximal mandible in mice. In: Developmental Biology. 2010 ; Vol. 344, No. 2. pp. 669-681.
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