Mesenteric visceral lipectomy using tissue liquefaction technology reverses insulin resistance and causes weight loss in baboons

Mark S. Andrew, Derek M. Huffman, Ernesto Rodriguez-Ayala, Noel N. Williams, Richard M. Peterson, Raul A. Bastarrachea

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Visceral obesity is associated with diabetogenic and atherogenic abnormalities, including insulin resistance and increased risk for cardiometabolic diseases and mortality. Rodent lipectomy studies have demonstrated a causal link between visceral fat and insulin resistance, yet human omentectomy studies have failed to replicate this metabolic benefit, perhaps owing to the inability to target the mesentery. Objectives: We aimed to demonstrate that safe and effective removal of mesenteric fat could be achieved in obese insulin-resistant baboons using tissue liquefaction technology. Setting: Southwest National Primate Research Center, San Antonio, Texas. Methods: Tissue liquefaction technology has been developed to enable mesenteric visceral lipectomy (MVL) to be safely performed without disturbing the integrity of surrounding nerves and vessels in the mesentary. After an initial MVL optimization study (n = 3), we then performed MVL (n = 4) or sham surgery (n = 2) in a cohort of insulin-resistant baboons, and the metabolic phenotype was assessed via hyperinsulinemic-euglycemic clamps at baseline and 6 weeks later. Results: MVL led to a 75% improvement in glucose disposal at 6-weeks follow-up (P =.01). Moreover, despite removing only an average of 430 g of mesenteric fat (~1% of total body mass), MVL led to a 14.4% reduction in total weight (P =.001). Thus, these data demonstrate that mesenteric fat can be safely targeted for removal by tissue liquefaction technology in a nonhuman primate, leading to substantial metabolic improvements, including reversal of insulin resistance and weight loss. Conclusions: These data provide the first demonstration of successful adipose tissue removal from the mesentery in a mammal. Importantly, we have demonstrated that when MVL is performed in obese, insulin-resistant baboons, insulin resistance is reversed, and significant weight loss occurs. Therefore, trials performing MVL in humans with abdominal obesity and related metabolic sequelae should be explored as a potential clinical tool to ameliorate insulin resistance and treat type 2 diabetes.

Original languageEnglish (US)
Pages (from-to)833-841
Number of pages9
JournalSurgery for Obesity and Related Diseases
Volume14
Issue number6
DOIs
StatePublished - Jun 1 2018

Fingerprint

Lipectomy
Papio
Insulin Resistance
Weight Loss
Technology
Mesentery
Abdominal Obesity
Fats
Insulin
Primates
Glucose Clamp Technique
Intra-Abdominal Fat
Type 2 Diabetes Mellitus
Adipose Tissue
Mammals
Rodentia
Phenotype
Weights and Measures
Glucose
Mortality

Keywords

  • Hypophagia
  • Insulin-resistant baboons
  • Mesenteric fat
  • Metabolic improvement
  • Tissue liquefaction technology

ASJC Scopus subject areas

  • Surgery

Cite this

Mesenteric visceral lipectomy using tissue liquefaction technology reverses insulin resistance and causes weight loss in baboons. / Andrew, Mark S.; Huffman, Derek M.; Rodriguez-Ayala, Ernesto; Williams, Noel N.; Peterson, Richard M.; Bastarrachea, Raul A.

In: Surgery for Obesity and Related Diseases, Vol. 14, No. 6, 01.06.2018, p. 833-841.

Research output: Contribution to journalArticle

Andrew, Mark S. ; Huffman, Derek M. ; Rodriguez-Ayala, Ernesto ; Williams, Noel N. ; Peterson, Richard M. ; Bastarrachea, Raul A. / Mesenteric visceral lipectomy using tissue liquefaction technology reverses insulin resistance and causes weight loss in baboons. In: Surgery for Obesity and Related Diseases. 2018 ; Vol. 14, No. 6. pp. 833-841.
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AU - Peterson, Richard M.

AU - Bastarrachea, Raul A.

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AB - Background: Visceral obesity is associated with diabetogenic and atherogenic abnormalities, including insulin resistance and increased risk for cardiometabolic diseases and mortality. Rodent lipectomy studies have demonstrated a causal link between visceral fat and insulin resistance, yet human omentectomy studies have failed to replicate this metabolic benefit, perhaps owing to the inability to target the mesentery. Objectives: We aimed to demonstrate that safe and effective removal of mesenteric fat could be achieved in obese insulin-resistant baboons using tissue liquefaction technology. Setting: Southwest National Primate Research Center, San Antonio, Texas. Methods: Tissue liquefaction technology has been developed to enable mesenteric visceral lipectomy (MVL) to be safely performed without disturbing the integrity of surrounding nerves and vessels in the mesentary. After an initial MVL optimization study (n = 3), we then performed MVL (n = 4) or sham surgery (n = 2) in a cohort of insulin-resistant baboons, and the metabolic phenotype was assessed via hyperinsulinemic-euglycemic clamps at baseline and 6 weeks later. Results: MVL led to a 75% improvement in glucose disposal at 6-weeks follow-up (P =.01). Moreover, despite removing only an average of 430 g of mesenteric fat (~1% of total body mass), MVL led to a 14.4% reduction in total weight (P =.001). Thus, these data demonstrate that mesenteric fat can be safely targeted for removal by tissue liquefaction technology in a nonhuman primate, leading to substantial metabolic improvements, including reversal of insulin resistance and weight loss. Conclusions: These data provide the first demonstration of successful adipose tissue removal from the mesentery in a mammal. Importantly, we have demonstrated that when MVL is performed in obese, insulin-resistant baboons, insulin resistance is reversed, and significant weight loss occurs. Therefore, trials performing MVL in humans with abdominal obesity and related metabolic sequelae should be explored as a potential clinical tool to ameliorate insulin resistance and treat type 2 diabetes.

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KW - Metabolic improvement

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