Menin interacts with the AP1 transcription factor JunD and represses JunD-activated transcription

Sunita K. Agarwal, Siradanahalli C. Guru, Christina Heppner, Michael R. Erdos, Regina M. Collins, Sylvia Y. Park, Suraj Saggar, Settara C. Chandrasekharappa, Francis S. Collins, Allen M. Spiegel, Stephen J. Marx, A. Lee Burns

Research output: Contribution to journalArticle

479 Citations (Scopus)

Abstract

MEN1 is a tumor suppressor gene that encodes a 610 amino acid nuclear protein (menin) of previously unknown function. Using a yeast two-hybrid screen with menin as the bait, we have identified the transcription factor JunD as a direct menin-interacting partner. Menin did not interact directly with other Jun and Fos family members. The menin-JunD interaction was confirmed in vitro and in vivo. Menin repressed transcriptional activation mediated by JunD fused to the Gal4 DNA-binding domain from a Gal4 responsive reporter, or by JunD from an AP1-responsive reporter. Several naturally occurring and clustered MEN1 missense mutations disrupted menin interaction with JunD. These observations suggest that menin's tumor suppressor function involves direct binding to JunD and inhibition of JunD activated transcription.

Original languageEnglish (US)
Pages (from-to)143-152
Number of pages10
JournalCell
Volume96
Issue number1
DOIs
StatePublished - Jan 8 1999
Externally publishedYes

Fingerprint

Multiple Endocrine Neoplasia Type 1
Transcription
Tumors
Transcription Factors
Missense Mutation
Nuclear Proteins
Tumor Suppressor Genes
Yeast
Transcriptional Activation
Genes
Yeasts
Chemical activation
Amino Acids
DNA
Neoplasms
Inhibition (Psychology)
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Agarwal, S. K., Guru, S. C., Heppner, C., Erdos, M. R., Collins, R. M., Park, S. Y., ... Lee Burns, A. (1999). Menin interacts with the AP1 transcription factor JunD and represses JunD-activated transcription. Cell, 96(1), 143-152. https://doi.org/10.1016/S0092-8674(00)80967-8

Menin interacts with the AP1 transcription factor JunD and represses JunD-activated transcription. / Agarwal, Sunita K.; Guru, Siradanahalli C.; Heppner, Christina; Erdos, Michael R.; Collins, Regina M.; Park, Sylvia Y.; Saggar, Suraj; Chandrasekharappa, Settara C.; Collins, Francis S.; Spiegel, Allen M.; Marx, Stephen J.; Lee Burns, A.

In: Cell, Vol. 96, No. 1, 08.01.1999, p. 143-152.

Research output: Contribution to journalArticle

Agarwal, SK, Guru, SC, Heppner, C, Erdos, MR, Collins, RM, Park, SY, Saggar, S, Chandrasekharappa, SC, Collins, FS, Spiegel, AM, Marx, SJ & Lee Burns, A 1999, 'Menin interacts with the AP1 transcription factor JunD and represses JunD-activated transcription', Cell, vol. 96, no. 1, pp. 143-152. https://doi.org/10.1016/S0092-8674(00)80967-8
Agarwal SK, Guru SC, Heppner C, Erdos MR, Collins RM, Park SY et al. Menin interacts with the AP1 transcription factor JunD and represses JunD-activated transcription. Cell. 1999 Jan 8;96(1):143-152. https://doi.org/10.1016/S0092-8674(00)80967-8
Agarwal, Sunita K. ; Guru, Siradanahalli C. ; Heppner, Christina ; Erdos, Michael R. ; Collins, Regina M. ; Park, Sylvia Y. ; Saggar, Suraj ; Chandrasekharappa, Settara C. ; Collins, Francis S. ; Spiegel, Allen M. ; Marx, Stephen J. ; Lee Burns, A. / Menin interacts with the AP1 transcription factor JunD and represses JunD-activated transcription. In: Cell. 1999 ; Vol. 96, No. 1. pp. 143-152.
@article{eca671d248d64747a2d337b1e9233eba,
title = "Menin interacts with the AP1 transcription factor JunD and represses JunD-activated transcription",
abstract = "MEN1 is a tumor suppressor gene that encodes a 610 amino acid nuclear protein (menin) of previously unknown function. Using a yeast two-hybrid screen with menin as the bait, we have identified the transcription factor JunD as a direct menin-interacting partner. Menin did not interact directly with other Jun and Fos family members. The menin-JunD interaction was confirmed in vitro and in vivo. Menin repressed transcriptional activation mediated by JunD fused to the Gal4 DNA-binding domain from a Gal4 responsive reporter, or by JunD from an AP1-responsive reporter. Several naturally occurring and clustered MEN1 missense mutations disrupted menin interaction with JunD. These observations suggest that menin's tumor suppressor function involves direct binding to JunD and inhibition of JunD activated transcription.",
author = "Agarwal, {Sunita K.} and Guru, {Siradanahalli C.} and Christina Heppner and Erdos, {Michael R.} and Collins, {Regina M.} and Park, {Sylvia Y.} and Suraj Saggar and Chandrasekharappa, {Settara C.} and Collins, {Francis S.} and Spiegel, {Allen M.} and Marx, {Stephen J.} and {Lee Burns}, A.",
year = "1999",
month = "1",
day = "8",
doi = "10.1016/S0092-8674(00)80967-8",
language = "English (US)",
volume = "96",
pages = "143--152",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - Menin interacts with the AP1 transcription factor JunD and represses JunD-activated transcription

AU - Agarwal, Sunita K.

AU - Guru, Siradanahalli C.

AU - Heppner, Christina

AU - Erdos, Michael R.

AU - Collins, Regina M.

AU - Park, Sylvia Y.

AU - Saggar, Suraj

AU - Chandrasekharappa, Settara C.

AU - Collins, Francis S.

AU - Spiegel, Allen M.

AU - Marx, Stephen J.

AU - Lee Burns, A.

PY - 1999/1/8

Y1 - 1999/1/8

N2 - MEN1 is a tumor suppressor gene that encodes a 610 amino acid nuclear protein (menin) of previously unknown function. Using a yeast two-hybrid screen with menin as the bait, we have identified the transcription factor JunD as a direct menin-interacting partner. Menin did not interact directly with other Jun and Fos family members. The menin-JunD interaction was confirmed in vitro and in vivo. Menin repressed transcriptional activation mediated by JunD fused to the Gal4 DNA-binding domain from a Gal4 responsive reporter, or by JunD from an AP1-responsive reporter. Several naturally occurring and clustered MEN1 missense mutations disrupted menin interaction with JunD. These observations suggest that menin's tumor suppressor function involves direct binding to JunD and inhibition of JunD activated transcription.

AB - MEN1 is a tumor suppressor gene that encodes a 610 amino acid nuclear protein (menin) of previously unknown function. Using a yeast two-hybrid screen with menin as the bait, we have identified the transcription factor JunD as a direct menin-interacting partner. Menin did not interact directly with other Jun and Fos family members. The menin-JunD interaction was confirmed in vitro and in vivo. Menin repressed transcriptional activation mediated by JunD fused to the Gal4 DNA-binding domain from a Gal4 responsive reporter, or by JunD from an AP1-responsive reporter. Several naturally occurring and clustered MEN1 missense mutations disrupted menin interaction with JunD. These observations suggest that menin's tumor suppressor function involves direct binding to JunD and inhibition of JunD activated transcription.

UR - http://www.scopus.com/inward/record.url?scp=0033534510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033534510&partnerID=8YFLogxK

U2 - 10.1016/S0092-8674(00)80967-8

DO - 10.1016/S0092-8674(00)80967-8

M3 - Article

VL - 96

SP - 143

EP - 152

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -