MEN1 gene analysis in sporadic adrenocortical neoplasms

Christina Heppner, Martin Reincke, Sunita K. Agarwal, Patricia Mora, Bruno Allolio, A. Lee Burns, Allen M. Spiegel, Stephen J. Marx

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Adrenocortical tumors occur as sporadic tumors, as part of the multiple endocrine neoplasia type 1 (MEN1) syndrome or as part of other hereditary disorders. We recently cloned the MEN1 gene, a tumor-suppressor gene located on chromosome 11q13. Subsequently, we showed that sequential sematic inactivation of both alleles of the MEN1 gene contributes to the development of some sporadic endocrine neoplasms (parathyroid, enteropancreatic neuroendocrine, bronchial carcinoid, and pituitary tumors). We now studied whether somatic inactivation of the MEN1 gene contributes to the pathogenesis of sporadic adrenocortical neoplasms. Seven adrenocortical carcinomas, 2 adrenocortical carcinoma cell lines, and 11 aldosterone-secreting, 8 cortisol-secreting, and 5 nonsecreting benign adrenocortical tumors were studied. Seven tumors (5 of 5 carcinomas, 2 of 21 nonsecreting benign adenomas; P < 0.001) exhibited loss of heterozygosity on 11q13. All 33 tumors and cell lines were screened for mutation throughout the MEN1 open-reading frame and adjacent splice junctions. None exhibited a mutation within the MEN1-coding region. We conclude that somatic MEN1 mutation within, the MEN1- coding region does not occur commonly in sporadic adrenocortical tumors, although the majority of adrenocortical carcinomas exhibit 11q13 loss of heterozygosity.

Original languageEnglish (US)
Pages (from-to)216-219
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume84
Issue number1
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Multiple Endocrine Neoplasia Type 1
Tumors
Genes
Adrenocortical Carcinoma
Neoplasms
Loss of Heterozygosity
Mutation
Cells
Multiple Endocrine Neoplasia
Parathyroid Neoplasms
Carcinoid Tumor
Pituitary Neoplasms
Chromosomes
Tumor Cell Line
Tumor Suppressor Genes
Aldosterone
Adenoma
Open Reading Frames
Hydrocortisone
Alleles

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Heppner, C., Reincke, M., Agarwal, S. K., Mora, P., Allolio, B., Burns, A. L., ... Marx, S. J. (1999). MEN1 gene analysis in sporadic adrenocortical neoplasms. Journal of Clinical Endocrinology and Metabolism, 84(1), 216-219. https://doi.org/10.1210/jc.84.1.216

MEN1 gene analysis in sporadic adrenocortical neoplasms. / Heppner, Christina; Reincke, Martin; Agarwal, Sunita K.; Mora, Patricia; Allolio, Bruno; Burns, A. Lee; Spiegel, Allen M.; Marx, Stephen J.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 84, No. 1, 1999, p. 216-219.

Research output: Contribution to journalArticle

Heppner, C, Reincke, M, Agarwal, SK, Mora, P, Allolio, B, Burns, AL, Spiegel, AM & Marx, SJ 1999, 'MEN1 gene analysis in sporadic adrenocortical neoplasms', Journal of Clinical Endocrinology and Metabolism, vol. 84, no. 1, pp. 216-219. https://doi.org/10.1210/jc.84.1.216
Heppner C, Reincke M, Agarwal SK, Mora P, Allolio B, Burns AL et al. MEN1 gene analysis in sporadic adrenocortical neoplasms. Journal of Clinical Endocrinology and Metabolism. 1999;84(1):216-219. https://doi.org/10.1210/jc.84.1.216
Heppner, Christina ; Reincke, Martin ; Agarwal, Sunita K. ; Mora, Patricia ; Allolio, Bruno ; Burns, A. Lee ; Spiegel, Allen M. ; Marx, Stephen J. / MEN1 gene analysis in sporadic adrenocortical neoplasms. In: Journal of Clinical Endocrinology and Metabolism. 1999 ; Vol. 84, No. 1. pp. 216-219.
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