Memory CD8+ T cells mediate antibacterial immunity via CCL3 activation of TNF/ROI+ phagocytes

Emilie Narni-Mancinelli, Laura Campisi, Delphine Bassand, Julie Cazareth, Pierre Gounon, Nicolas Glaichenhaus, Gregoire Lauvau

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Cytolysis, interferon γ and tumor necrosis factor (TNF) α secretion are major effector mechanisms of memory CD8+ T cells that are believed to be required for immunological protection in vivo. By using mutants of the intracellular bacterium Listeria monocytogenes, we found that none of these effector activities is sufficient to protect against secondary infection with wild-type (WT) bacteria. We demonstrated that CCL3 derived from reactivated memory CD8+ T cells is required for efficient killing of WT bacteria. CCL3 induces a rapid TNF-α secretion by innate inflammatory mononuclear phagocytic cells (MPCs), which further promotes the production of radical oxygen intermediates (ROIs) by both MPCs and neutrophils. ROI generation is the final bactericidal mechanism involved in L. monocytogenes clearance. These results therefore uncover two levels of regulation of the antibacterial secondary protective response: (a) an antigen-dependent phase in which memory CD8+ T cells are reactivated and control the activation of the innate immune system, and (b) an antigen-independent phase in which the MPCs coordinate innate immunity and promote the bactericidal effector activities. In this context, CCL3-secreting memory CD8+ T cells are able to mediate "bystander" killing of an unrelated pathogen upon antigen-specific reactivation, a mechanism that may be important for the design of therapeutic vaccines. JEM

Original languageEnglish (US)
Pages (from-to)2075-2087
Number of pages13
JournalJournal of Experimental Medicine
Volume204
Issue number9
DOIs
StatePublished - Sep 3 2007
Externally publishedYes

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Phagocytes
Immunity
Reactive Oxygen Species
Tumor Necrosis Factor-alpha
T-Lymphocytes
Listeria monocytogenes
Bacteria
Antigens
Coinfection
Innate Immunity
Interferons
Immune System
Neutrophils
Vaccines
Therapeutics

ASJC Scopus subject areas

  • Immunology

Cite this

Narni-Mancinelli, E., Campisi, L., Bassand, D., Cazareth, J., Gounon, P., Glaichenhaus, N., & Lauvau, G. (2007). Memory CD8+ T cells mediate antibacterial immunity via CCL3 activation of TNF/ROI+ phagocytes. Journal of Experimental Medicine, 204(9), 2075-2087. https://doi.org/10.1084/jem.20070204

Memory CD8+ T cells mediate antibacterial immunity via CCL3 activation of TNF/ROI+ phagocytes. / Narni-Mancinelli, Emilie; Campisi, Laura; Bassand, Delphine; Cazareth, Julie; Gounon, Pierre; Glaichenhaus, Nicolas; Lauvau, Gregoire.

In: Journal of Experimental Medicine, Vol. 204, No. 9, 03.09.2007, p. 2075-2087.

Research output: Contribution to journalArticle

Narni-Mancinelli, E, Campisi, L, Bassand, D, Cazareth, J, Gounon, P, Glaichenhaus, N & Lauvau, G 2007, 'Memory CD8+ T cells mediate antibacterial immunity via CCL3 activation of TNF/ROI+ phagocytes', Journal of Experimental Medicine, vol. 204, no. 9, pp. 2075-2087. https://doi.org/10.1084/jem.20070204
Narni-Mancinelli E, Campisi L, Bassand D, Cazareth J, Gounon P, Glaichenhaus N et al. Memory CD8+ T cells mediate antibacterial immunity via CCL3 activation of TNF/ROI+ phagocytes. Journal of Experimental Medicine. 2007 Sep 3;204(9):2075-2087. https://doi.org/10.1084/jem.20070204
Narni-Mancinelli, Emilie ; Campisi, Laura ; Bassand, Delphine ; Cazareth, Julie ; Gounon, Pierre ; Glaichenhaus, Nicolas ; Lauvau, Gregoire. / Memory CD8+ T cells mediate antibacterial immunity via CCL3 activation of TNF/ROI+ phagocytes. In: Journal of Experimental Medicine. 2007 ; Vol. 204, No. 9. pp. 2075-2087.
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