Membrane protein transport between the endoplasmic reticulum and the Golgi in tobacco leaves is energy dependent but cytoskeleton independent: Evidence from selective photobleaching

Federica Brandizzi, Erik L. Snapp, Alison G. Roberts, Jennifer Lippincott-Schwartz, Chris Hawes

Research output: Contribution to journalArticle

253 Citations (Scopus)

Abstract

The mechanisms that control protein transport between the endoplasmic reticulum (ER) and the Golgi apparatus are poorly characterized in plants. Here, we examine in tobacco leaves the structural relationship between Golgi and ER membranes using electron microscopy and demonstrate that Golgi membranes contain elements that are in close association and/or in direct contact with the ER. We further visualized protein trafficking between the ER and the Golgi using Golgi marker proteins tagged with green fluorescent protein. Using photobleaching techniques, we showed that Golgi membrane markers constitutively cycle to and from the Golgi in an energy-dependent and N-ethylmaleimide-sensitive manner. We found that membrane protein transport toward the Golgi occurs independently of the cytoskeleton and does not require the Golgi to be motile along the surface of the ER. Brefeldin A treatment blocked forward trafficking of Golgi proteins before their redistribution into the ER. Our results indicate that in plant cells, the Golgi apparatus is a dynamic membrane system whose components continuously traffic via membrane trafficking pathways regulated by brefeldin A- and N-ethylmaleimide-sensitive machinery.

Original languageEnglish (US)
Pages (from-to)1293-1309
Number of pages17
JournalPlant Cell
Volume14
Issue number6
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Photobleaching
photobleaching
Membrane Transport Proteins
Tobacco
protein transport
cytoskeleton
Cytoskeleton
membrane proteins
Endoplasmic Reticulum
endoplasmic reticulum
tobacco
Membranes
energy
Protein Transport
Brefeldin A
Ethylmaleimide
brefeldin A
leaves
Golgi Apparatus
Golgi apparatus

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Membrane protein transport between the endoplasmic reticulum and the Golgi in tobacco leaves is energy dependent but cytoskeleton independent : Evidence from selective photobleaching. / Brandizzi, Federica; Snapp, Erik L.; Roberts, Alison G.; Lippincott-Schwartz, Jennifer; Hawes, Chris.

In: Plant Cell, Vol. 14, No. 6, 2002, p. 1293-1309.

Research output: Contribution to journalArticle

Brandizzi, Federica ; Snapp, Erik L. ; Roberts, Alison G. ; Lippincott-Schwartz, Jennifer ; Hawes, Chris. / Membrane protein transport between the endoplasmic reticulum and the Golgi in tobacco leaves is energy dependent but cytoskeleton independent : Evidence from selective photobleaching. In: Plant Cell. 2002 ; Vol. 14, No. 6. pp. 1293-1309.
@article{36cb16ebbfd2439a85c36bba6e93cad2,
title = "Membrane protein transport between the endoplasmic reticulum and the Golgi in tobacco leaves is energy dependent but cytoskeleton independent: Evidence from selective photobleaching",
abstract = "The mechanisms that control protein transport between the endoplasmic reticulum (ER) and the Golgi apparatus are poorly characterized in plants. Here, we examine in tobacco leaves the structural relationship between Golgi and ER membranes using electron microscopy and demonstrate that Golgi membranes contain elements that are in close association and/or in direct contact with the ER. We further visualized protein trafficking between the ER and the Golgi using Golgi marker proteins tagged with green fluorescent protein. Using photobleaching techniques, we showed that Golgi membrane markers constitutively cycle to and from the Golgi in an energy-dependent and N-ethylmaleimide-sensitive manner. We found that membrane protein transport toward the Golgi occurs independently of the cytoskeleton and does not require the Golgi to be motile along the surface of the ER. Brefeldin A treatment blocked forward trafficking of Golgi proteins before their redistribution into the ER. Our results indicate that in plant cells, the Golgi apparatus is a dynamic membrane system whose components continuously traffic via membrane trafficking pathways regulated by brefeldin A- and N-ethylmaleimide-sensitive machinery.",
author = "Federica Brandizzi and Snapp, {Erik L.} and Roberts, {Alison G.} and Jennifer Lippincott-Schwartz and Chris Hawes",
year = "2002",
doi = "10.1105/tpc.001586",
language = "English (US)",
volume = "14",
pages = "1293--1309",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "6",

}

TY - JOUR

T1 - Membrane protein transport between the endoplasmic reticulum and the Golgi in tobacco leaves is energy dependent but cytoskeleton independent

T2 - Evidence from selective photobleaching

AU - Brandizzi, Federica

AU - Snapp, Erik L.

AU - Roberts, Alison G.

AU - Lippincott-Schwartz, Jennifer

AU - Hawes, Chris

PY - 2002

Y1 - 2002

N2 - The mechanisms that control protein transport between the endoplasmic reticulum (ER) and the Golgi apparatus are poorly characterized in plants. Here, we examine in tobacco leaves the structural relationship between Golgi and ER membranes using electron microscopy and demonstrate that Golgi membranes contain elements that are in close association and/or in direct contact with the ER. We further visualized protein trafficking between the ER and the Golgi using Golgi marker proteins tagged with green fluorescent protein. Using photobleaching techniques, we showed that Golgi membrane markers constitutively cycle to and from the Golgi in an energy-dependent and N-ethylmaleimide-sensitive manner. We found that membrane protein transport toward the Golgi occurs independently of the cytoskeleton and does not require the Golgi to be motile along the surface of the ER. Brefeldin A treatment blocked forward trafficking of Golgi proteins before their redistribution into the ER. Our results indicate that in plant cells, the Golgi apparatus is a dynamic membrane system whose components continuously traffic via membrane trafficking pathways regulated by brefeldin A- and N-ethylmaleimide-sensitive machinery.

AB - The mechanisms that control protein transport between the endoplasmic reticulum (ER) and the Golgi apparatus are poorly characterized in plants. Here, we examine in tobacco leaves the structural relationship between Golgi and ER membranes using electron microscopy and demonstrate that Golgi membranes contain elements that are in close association and/or in direct contact with the ER. We further visualized protein trafficking between the ER and the Golgi using Golgi marker proteins tagged with green fluorescent protein. Using photobleaching techniques, we showed that Golgi membrane markers constitutively cycle to and from the Golgi in an energy-dependent and N-ethylmaleimide-sensitive manner. We found that membrane protein transport toward the Golgi occurs independently of the cytoskeleton and does not require the Golgi to be motile along the surface of the ER. Brefeldin A treatment blocked forward trafficking of Golgi proteins before their redistribution into the ER. Our results indicate that in plant cells, the Golgi apparatus is a dynamic membrane system whose components continuously traffic via membrane trafficking pathways regulated by brefeldin A- and N-ethylmaleimide-sensitive machinery.

UR - http://www.scopus.com/inward/record.url?scp=0035983848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035983848&partnerID=8YFLogxK

U2 - 10.1105/tpc.001586

DO - 10.1105/tpc.001586

M3 - Article

C2 - 12084828

AN - SCOPUS:0035983848

VL - 14

SP - 1293

EP - 1309

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 6

ER -