Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes

Sandra M. Cordo, Ayelén Valko, Guadalupe M. Martinez, Nélida A. Candurra

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Arenavirus morphogenesis and budding occurs at cellular plasma membrane; however, the nature of membrane assembly sites remains poorly understood. In this study we examined the effect of different cholesterol-lowering agents on Junín virus (JUNV) multiplication. We found that cholesterol cell depletion reduced JUNV glycoproteins (GPs) membrane expression and virus budding. Analysis of membrane protein insolubility in Triton X-100 suggested that JUNV GPs associate with cholesterol enriched membranes. Rafts dissociation conditions as warm detergent extraction and cholesterol removal by methyl-β-cyclodextrin compound showed to impair GPs cholesterol enriched membrane association. Analysis of GPs transfected cells showed similar results suggesting that membrane raft association is independent of other viral proteins.

Original languageEnglish (US)
Pages (from-to)912-917
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume430
Issue number3
DOIs
StatePublished - Jan 18 2013

Keywords

  • Cholesterol
  • Junín virus
  • Membrane rafts

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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