Membrane lipid organization is critical for human neutrophil polarization

Lynda M. Pierini, Robert J. Eddy, Michele Fuortes, Stéphanie Seveau, Carlo Casulo, Frederick R. Maxfield

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

In response to chemoattractants neutrophils extend an actin-rich pseudopod, which imparts morphological polarity and is required for migration. Even when stimulated by an isotropic bath of chemoattractant, neutrophils exhibit persistent polarization and continued lamellipod formation at the front, suggesting that the cells establish an internal polarity. In this report, we show that perturbing lipid organization by depleting plasma membrane cholesterol levels reversibly inhibits cell polarization and migration. Among other receptor-mediated responses, β2 integrin up-regulation was unaffected, and initial calcium mobilization was only partially reduced by cholesterol depletion, indicating that this treatment did not abrogate initial receptor-mediated signal transduction. Interestingly, cholesterol depletion did not prevent initial activation of the GTPase Rac or an initial burst of actin polymerization, but rather it inhibited prolonged activation of Rac and sustained actin polymerization. Collectively, these findings support a model in which the plasma membrane is organized into domains that aid in amplifying the chemoattractant gradient and maintaining cell polarization.

Original languageEnglish (US)
Pages (from-to)10831-10841
Number of pages11
JournalJournal of Biological Chemistry
Volume278
Issue number12
DOIs
StatePublished - Mar 21 2003
Externally publishedYes

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Chemotactic Factors
Membrane Lipids
Actins
Neutrophils
Cholesterol
Cell membranes
Polarization
Polymerization
Chemical activation
Cell Membrane
Signal transduction
Pseudopodia
GTP Phosphohydrolases
Baths
Integrins
Cell Movement
Signal Transduction
Up-Regulation
Calcium
Lipids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pierini, L. M., Eddy, R. J., Fuortes, M., Seveau, S., Casulo, C., & Maxfield, F. R. (2003). Membrane lipid organization is critical for human neutrophil polarization. Journal of Biological Chemistry, 278(12), 10831-10841. https://doi.org/10.1074/jbc.M212386200

Membrane lipid organization is critical for human neutrophil polarization. / Pierini, Lynda M.; Eddy, Robert J.; Fuortes, Michele; Seveau, Stéphanie; Casulo, Carlo; Maxfield, Frederick R.

In: Journal of Biological Chemistry, Vol. 278, No. 12, 21.03.2003, p. 10831-10841.

Research output: Contribution to journalArticle

Pierini, LM, Eddy, RJ, Fuortes, M, Seveau, S, Casulo, C & Maxfield, FR 2003, 'Membrane lipid organization is critical for human neutrophil polarization', Journal of Biological Chemistry, vol. 278, no. 12, pp. 10831-10841. https://doi.org/10.1074/jbc.M212386200
Pierini, Lynda M. ; Eddy, Robert J. ; Fuortes, Michele ; Seveau, Stéphanie ; Casulo, Carlo ; Maxfield, Frederick R. / Membrane lipid organization is critical for human neutrophil polarization. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 12. pp. 10831-10841.
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