Recombinant cDNAs encoding the α-subunits of Gi1, Gi2, Gi3, GO and GS were transfected into COS cells with the pCD-PS mammalian expression vector. Expression of each Gα was verified using subtype-specific peptide antisera on immunoblots. Quantitative immunoblotting of α and β subunits indicated: i) that there was no change in expression of endogenous β subunits, and ii) overexpression of α subunits could achieve a ratio of α:β > 25:1. Despite the excess of α over β, the Gα subunits were found predominantly in the membrane fraction. The results demonstrate that Gα subunits can attach to the membrane independently of βγ subunits.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Oct 16 1989|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology