Abstract
Recombinant cDNAs encoding the α-subunits of Gi1, Gi2, Gi3, GO and GS were transfected into COS cells with the pCD-PS mammalian expression vector. Expression of each Gα was verified using subtype-specific peptide antisera on immunoblots. Quantitative immunoblotting of α and β subunits indicated: i) that there was no change in expression of endogenous β subunits, and ii) overexpression of α subunits could achieve a ratio of α:β > 25:1. Despite the excess of α over β, the Gα subunits were found predominantly in the membrane fraction. The results demonstrate that Gα subunits can attach to the membrane independently of βγ subunits.
Original language | English (US) |
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Pages (from-to) | 46-53 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 164 |
Issue number | 1 |
DOIs | |
State | Published - Oct 16 1989 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology