Melanization of Cryptococcus neoformans in murine infection

Joshua D. Nosanchuk, Philippe Valadon, Marta Feldmesser, Arturo Casadevall

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Cryptococcus neoformans is a fungus that is pathogenic in humans and that can produce melanin in vitro. Melanization is associated with virulence, but there is no evidence that melanin is made during infection. Melanins are difficult to study because they are amorphous and insoluble. Melanin-binding peptides from a phage display library were used to demonstrate that C. neoformans makes melanin-like compounds in tissue. Melanin-binding peptides were characterized by a high proportion of positively charged and aromatic residues. Two other methods, demonstration of an antibody response to melanin in mice infected with C. neoformans and analysis of yeast cell walls in infected tissue by light microscopy, were used to support these findings. The demonstration that C. neoformans melanizes in tissue has important implications for pathogenesis and drug discovery.

Original languageEnglish (US)
Pages (from-to)745-750
Number of pages6
JournalMolecular and Cellular Biology
Volume19
Issue number1
StatePublished - Jan 1999

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Cryptococcus neoformans
Melanins
Infection
Peptide Library
Drug Discovery
Cell Wall
Antibody Formation
Virulence
Microscopy
Fungi
Yeasts
Light
Peptides

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Nosanchuk, J. D., Valadon, P., Feldmesser, M., & Casadevall, A. (1999). Melanization of Cryptococcus neoformans in murine infection. Molecular and Cellular Biology, 19(1), 745-750.

Melanization of Cryptococcus neoformans in murine infection. / Nosanchuk, Joshua D.; Valadon, Philippe; Feldmesser, Marta; Casadevall, Arturo.

In: Molecular and Cellular Biology, Vol. 19, No. 1, 01.1999, p. 745-750.

Research output: Contribution to journalArticle

Nosanchuk, JD, Valadon, P, Feldmesser, M & Casadevall, A 1999, 'Melanization of Cryptococcus neoformans in murine infection', Molecular and Cellular Biology, vol. 19, no. 1, pp. 745-750.
Nosanchuk JD, Valadon P, Feldmesser M, Casadevall A. Melanization of Cryptococcus neoformans in murine infection. Molecular and Cellular Biology. 1999 Jan;19(1):745-750.
Nosanchuk, Joshua D. ; Valadon, Philippe ; Feldmesser, Marta ; Casadevall, Arturo. / Melanization of Cryptococcus neoformans in murine infection. In: Molecular and Cellular Biology. 1999 ; Vol. 19, No. 1. pp. 745-750.
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