Melanin as a potential cryptococcal defence against microbicidal proteins

Tamara L. Doering, Joshua D. Nosanchuk, W. K. Roberts, A. Casadevall

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Cryptococcus neoformans is an important fungal pathogen that synthesizes melanin when grown in the presence of phenolic substrates. The ability of C. neoformans to produce melanin is associated with virulence, but the specific role of melanin in the pathogenesis of infection is not clear. In this study the ability of C. neoformans melanin to bind proteins and protect against microbicidal peptides was investigated. Melanin was shown to bind a variety of proteins of fungal and mammalian origin. Melanin-protein interactions were dependent on the pH of the solution and on the amount of protein and melanin present. Melanized cells were less susceptible to killing by three microbicidal peptides: a defensin, a protegrin, and a magainin. Incubation of the microbicidal peptides with melanin particles, followed by removal of the melanin, reduced or abolished fungicidal activity, demonstrating interactions between peptides and melanin. The ability of melanin to bind proteins and to protect against microbicidal peptides suggests a protective function for melanin, whereby it sequesters microbicidal peptides and abrogates their activity.

Original languageEnglish (US)
Pages (from-to)175-181
Number of pages7
JournalMedical Mycology
Volume37
Issue number3
DOIs
StatePublished - Jun 1999

Fingerprint

Melanins
melanin
Proteins
proteins
peptides
Peptides
Cryptococcus neoformans
Magainins
fungal proteins
Defensins
Fungal Proteins
Virulence
virulence
pathogenesis

Keywords

  • Cryptococcus neoformans
  • Defensin
  • Magainin
  • Melanin
  • Protegrin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • veterinary(all)
  • Microbiology

Cite this

Melanin as a potential cryptococcal defence against microbicidal proteins. / Doering, Tamara L.; Nosanchuk, Joshua D.; Roberts, W. K.; Casadevall, A.

In: Medical Mycology, Vol. 37, No. 3, 06.1999, p. 175-181.

Research output: Contribution to journalArticle

Doering, Tamara L. ; Nosanchuk, Joshua D. ; Roberts, W. K. ; Casadevall, A. / Melanin as a potential cryptococcal defence against microbicidal proteins. In: Medical Mycology. 1999 ; Vol. 37, No. 3. pp. 175-181.
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