Mechanisms of platelet-neutrophil interactions in sepsis

Darryl Adler, Linda A. Kirschenbaum, Mark E. Astiz, Dhanonjoy C. Saha, Eric C. Rackow

Research output: Contribution to journalArticle

Abstract

Introduction: Platelet-neutrophil (P-PMN) interactions may contribute to impaired microvascular blood flow in severe sepsis (S). We examined whether blocking adhesive molecules mediating P-PMN interactions attenuates S induced P-PMN interactions. Methods: P's, PMN's, and normal platelet poor plasma (NPPP) were isolated from 12 normal subjects. Platelet poor plasma was isolated from 12 patients with S (SPPP). Blocking monoclonal antibodies (mAb) were added to desired cell lines after incubation with SPPP and before coincubation of PMN and P. The following mAb's were used: anti-CD41 recognizes the P complex GPIIb-IIIa; anti-CD62P blocks P-selectin on P; anti-CD11a/LFA blocks ICAM 2 on PMN; anti-CD11b and anti-CD18 block receptors on PMN. Cell suspensions were infused at 1 ml/m through 5 u pore filters and the maximal point of the pressure time curve, Pi (mm Hg), was recorded. P-PMN interactions were assessed using fluorecscein conjugated mAb to activated CD63 P receptors. The mean intensity of mAb fluorescence (MF) of the PMN subgroup was used as an index of P-PMN adhesion. Data are expressed as mean ± SE,*p

Original languageEnglish (US)
JournalCritical Care Medicine
Volume27
Issue number12 SUPPL.
StatePublished - 1999
Externally publishedYes

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Sepsis
Neutrophils
Blood Platelets
Monoclonal Antibodies
Blocking Antibodies
P-Selectin
Adhesives
Suspensions
Fluorescence
Pressure
Cell Line

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Adler, D., Kirschenbaum, L. A., Astiz, M. E., Saha, D. C., & Rackow, E. C. (1999). Mechanisms of platelet-neutrophil interactions in sepsis. Critical Care Medicine, 27(12 SUPPL.).

Mechanisms of platelet-neutrophil interactions in sepsis. / Adler, Darryl; Kirschenbaum, Linda A.; Astiz, Mark E.; Saha, Dhanonjoy C.; Rackow, Eric C.

In: Critical Care Medicine, Vol. 27, No. 12 SUPPL., 1999.

Research output: Contribution to journalArticle

Adler, D, Kirschenbaum, LA, Astiz, ME, Saha, DC & Rackow, EC 1999, 'Mechanisms of platelet-neutrophil interactions in sepsis', Critical Care Medicine, vol. 27, no. 12 SUPPL..
Adler D, Kirschenbaum LA, Astiz ME, Saha DC, Rackow EC. Mechanisms of platelet-neutrophil interactions in sepsis. Critical Care Medicine. 1999;27(12 SUPPL.).
Adler, Darryl ; Kirschenbaum, Linda A. ; Astiz, Mark E. ; Saha, Dhanonjoy C. ; Rackow, Eric C. / Mechanisms of platelet-neutrophil interactions in sepsis. In: Critical Care Medicine. 1999 ; Vol. 27, No. 12 SUPPL.
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AU - Adler, Darryl

AU - Kirschenbaum, Linda A.

AU - Astiz, Mark E.

AU - Saha, Dhanonjoy C.

AU - Rackow, Eric C.

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